anti-Human RPS6KB1 antibody for Enzyme Immunoassay

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • XPKAbeta
  • pkacb
  • 10539
  • 7084
  • CG10539
  • DS6K
  • Dmel\\CG10539
  • Dp70S6k
  • Dp70[s6k]
  • Dp70s6k
  • S6k
  • dS6K
  • dS6k
  • dp70[S6k]
  • dp70s6k
  • dps6k
  • ds6k
  • fs(3)07084
  • l(3)07084
  • p70 S6K
  • p70/S6K
  • p70S6K
  • p70[S6 kinase]
  • p70[S6K]
  • p70[S6k]
  • p70[S6kinase]
  • p70s6K
  • s6k
  • s6k11
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • p70s6k-A
  • p70-s6k
  • ps6k
  • rps6kb1
  • rps6kb1-A
  • s6K1
  • stk14a
  • fc51h01
  • wu:fc51h01
  • zgc:55713
  • ribosomal protein S6 kinase B1
  • protein kinase cAMP-activated catalytic subunit beta
  • Ribosomal protein S6 kinase
  • ribosomal protein S6 kinase, polypeptide 1
  • ribosomal protein S6 kinase B1 L homeolog
  • ribosomal protein S6 kinase B1 S homeolog
  • ribosomal protein S6 kinase b, polypeptide 1b
  • RPS6KB1
  • prkacb
  • S6k
  • Rps6kb1
  • rps6kb1.L
  • rps6kb1.S
  • rps6kb1b
Epitope
pSer418
108
39
34
29
29
21
21
20
18
18
17
17
15
15
15
14
14
12
12
10
10
9
8
6
6
6
6
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
700
442
419
73
54
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
627
53
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
549
282
263
151
127
104
65
42
22
20
17
11
6
2
1
1
1
1
Supplier
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Catalog No. ABIN358318
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5552808 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal 0
1 ABIN5551331 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5611016 EIA WB Rabbit Ig Fraction Ser404 Log in to see Polyclonal 0
1 ABIN5611015 EIA IHC (p) WB Rabbit Ig Fraction Ser424 Log in to see Polyclonal 0
1 ABIN1108904 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5G9 2
1 ABIN303144 EIA IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pSer418
(108), (39), (34), (29), (29), (21), (21), (20), (18), (18), (17), (17), (15), (15), (15), (14), (14), (12), (12), (10), (10), (9), (8), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(700), (442), (419), (73), (54), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(627), (53), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(549), (282), (263), (151), (127), (104), (65), (42), (22), (20), (17), (11), (6), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 antibody (ABIN358318) Images
Specificity This antibody detects RPS6KB1 pSer418. Predicted to cross react with Mouse and Rat (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser418 of human RPS6KB1.
Isotype Ig Fraction

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Antigen
Alternative Name RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1
Molecular Weight 59009 Da
Gene ID 6198, 9606
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RPS6KB1 antibody (ABIN358318)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Background publications

Adem, Soderberg, Hafner, Reynolds, Slezak, Sinclair, Sellers, Schaid, Couch, Hartmann, Jenkins: "ERBB2, TBX2, RPS6KB1, and MYC alterations in breast tissues of BRCA1 and BRCA2 mutation carriers." in: Genes, chromosomes & cancer, Vol. 41, Issue 1, pp. 1-11, 2004

Suzuki, Yamashita, Shirota, Sakakibara, Chiba, Mizushima-Sugano, Nakai, Sugano: "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions." in: Genome research, Vol. 14, Issue 9, pp. 1711-8, 2004

Gomez-Cambronero, Frye, Baumann: "Ribosomal p70S6K basal activity increases upon induction of differentiation of myelomonocytic leukemic cell lines HL60, AML14 and MPD." in: Leukemia research, Vol. 28, Issue 7, pp. 755-62, 2004

Raught, Peiretti, Gingras, Livingstone, Shahbazian, Mayeur, Polakiewicz, Sonenberg, Hershey: "Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases." in: The EMBO journal, Vol. 23, Issue 8, pp. 1761-9, 2004

Miyakawa, Tsushima, Murakami, Wakai, Isozaki, Takano: "Increased expression of phosphorylated p70S6 kinase and Akt in papillary thyroid cancer tissues." in: Endocrine journal, Vol. 50, Issue 1, pp. 77-83, 2003

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 antibody (ABIN358318) back to top
Supplier Images
 image for anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer418) antibody (ABIN358318) anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer418) antibody