anti-Rat (Rattus) CD28 antibody for ELISA

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Epitope
pTyr218
17
16
15
12
11
11
11
7
7
6
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
568
392
95
49
44
43
43
36
16
7
6
2
2
2
2
1
1
1
Host
Rabbit
556
170
98
59
51
31
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
111
91
72
58
22
19
13
13
11
10
9
9
9
9
8
8
8
8
8
8
8
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
797
265
177
158
148
143
126
124
85
52
48
26
13
11
9
5
3
3
3
3
2
2
2
2
1
1
1
1
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Catalog No. ABIN6255852
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5506225 ELISA Rabbit IgG AA 1-149 Log in to see Polyclonal 0

General

Antigen CD28 Antibodies
Epitope pTyr218
(17), (16), (15), (12), (11), (11), (11), (7), (7), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(568), (392), (95), (49), (44), (43), (43), (36), (16), (7), (6), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(556), (170), (98), (59), (51), (31), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(111), (91), (72), (58), (22), (19), (13), (13), (11), (10), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(797), (265), (177), (158), (148), (143), (126), (124), (85), (52), (48), (26), (13), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Phospho-CD28 (Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human Phospho-CD28 (Tyr218)
Isotype IgG
Plasmids, Primers & others

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background

Description: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Gene: CD28

Molecular Weight 25 kDa
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes WB 1:1000-3000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months