anti-Human CD74 antibody for Blocking Reagent

Recommended CD74 Antibody

Antigen
HLA-DR-gamma (CD74) Antibodies
  • DHLAG
  • HLADG
  • II
  • Ia-GAMMA
  • CLIP
  • Ii
  • INVG34
  • Iclp-1
  • iclp1
  • wu:fb11f09
  • wu:fb12a01
  • wu:fb13d06
  • wu:fk94e07
  • CD74 molecule
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain a
  • CD74
  • Cd74
  • cd74a
Reactivity
Human
535
121
31
29
Host
Mouse
453
105
10
2
Clonality
Monoclonal
Conjugate
This CD74 antibody is un-conjugated
36
32
25
20
19
16
14
9
9
9
9
9
9
9
9
9
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
348
340
234
136
99
96
64
63
60
58
16
13
10
2
2
2
2
2
1
1
Options

Available images

Catalog No. ABIN2664819
Contact our Customer Service for availability and price in your country.

General

Antigen HLA-DR-gamma (CD74) Antibodies
Reactivity Human
(535), (121), (31), (29)
Host Mouse
(453), (105), (10), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD74 antibody is un-conjugated
(36), (32), (25), (20), (19), (16), (14), (9), (9), (9), (9), (9), (9), (9), (9), (9), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(348), (340), (234), (136), (99), (96), (64), (63), (60), (58), (16), (13), (10), (2), (2), (2), (2), (2), (1), (1)
Pubmed 7 references available
Supplier

Product Details anti-CD74 Antibody

Target Details CD74 Application Details Handling References for anti-CD74 antibody (ABIN2664819) Images
Cross-Reactivity Baboon, Rhesus Monkey
Purification The antibody was purified by affinity chromatography.
Clone LN2
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details CD74

Product Details anti-CD74 Antibody Application Details Handling References for anti-CD74 antibody (ABIN2664819) Images back to top
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Background CD74 is a type II transmembrane glycoprotein also known as MHC class II associated invariant chain, invariant chain, Ii, MHC class II chaperone, and MIF receptor. CD74 exists in four isoforms with molecular masses of 33, 35, 41, and 43 kD, depending on genetic splicing. CD74 is primarily expressed on antigen presenting cells, including B cells, monocytes/macrophages, dendritic cells, and Langerhans cells. It is also expressed by activated T cells and activated endothelial and epithelial cells as well as carcinomas of lung, renal, gastric and thymic origin. The primary function of CD74 is intracellular sorting of MHC class II molecules and regulation of exogenous peptide loading onto MHC class II. It is also involved in the modulation of B cell differentiation and positive selection of CD4+ T cells. It has been reported that CD74 binds MIF (macrophage migration inhibitory factor) and signals through CD44 to regulate innate and adaptive immunity. It is also reported that H. pylori infection occurs through urease B binding of CD74 on gastric epithelial cells, inducing gastric epithelial cell apoptosis, NF-κB activation, and IL-8 production.
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints

Application Details

Product Details anti-CD74 Antibody Target Details CD74 Handling References for anti-CD74 antibody (ABIN2664819) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD74 Antibody Target Details CD74 Application Details References for anti-CD74 antibody (ABIN2664819) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD74 antibody (ABIN2664819)

Product Details anti-CD74 Antibody Target Details CD74 Application Details Handling Images back to top
Background publications

Beswick, Pinchuk, Minch, Suarez, Sierra, Yamaoka, Reyes: "The Helicobacter pylori urease B subunit binds to CD74 on gastric epithelial cells and induces NF-kappaB activation and interleukin-8 production." in: Infection and immunity, Vol. 74, Issue 2, pp. 1148-55, 2006

Shi, Leng, Wang, Wang, Du, Li, McDonald, Chen, Murphy, Lolis, Noble, Knudson, Bucala: "CD44 is the signaling component of the macrophage migration inhibitory factor-CD74 receptor complex." in: Immunity, Vol. 25, Issue 4, pp. 595-606, 2006

Leng, Metz, Fang, Xu, Donnelly, Baugh, Delohery, Chen, Mitchell, Bucala: "MIF signal transduction initiated by binding to CD74." in: The Journal of experimental medicine, Vol. 197, Issue 11, pp. 1467-76, 2003

Moldenhauer, Henne, Karhausen, Möller: "Surface-expressed invariant chain (CD74) is required for internalization of human leucocyte antigen-DR molecules to early endosomal compartments." in: Immunology, Vol. 96, Issue 3, pp. 473-84, 1999

Wraight, van Endert, Möller, Lipp, Ling, MacLennan, Koch, Moldenhauer: "Human major histocompatibility complex class II invariant chain is expressed on the cell surface." in: The Journal of biological chemistry, Vol. 265, Issue 10, pp. 5787-92, 1990

Marder, Variakojis, Silver, Epstein: "Immunohistochemical analysis of human lymphomas with monoclonal antibodies to B cell and Ia antigens reactive in paraffin sections." in: Laboratory investigation, a journal of technical methods and pathology, Vol. 52, Issue 5, pp. 497-504, 1985

Epstein, Marder, Winter, Fox: "Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 1028-36, 1984

Images

Product Details anti-CD74 Antibody Target Details CD74 Application Details Handling References for anti-CD74 antibody (ABIN2664819) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HLA-DR-gamma (CD74) antibody (ABIN2664819) anti-HLA-DR-gamma (CD74) antibody