anti-Human BRISC and BRCA1 A Complex Member 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BRISC and BRCA1 A Complex Member 1 Antibody (supplied by: Log in to see )

Antigen
BRISC and BRCA1 A Complex Member 1 (BABAM1) Antibodies
  • merit40
  • nba1
  • zgc:100909
  • c19orf62
  • C19orf62
  • MERIT40
  • NBA1
  • C7H19orf62
  • 5430437P03Rik
  • Merit40
  • BRISC and BRCA1 A complex member 1
  • BRISC and BRCA1 A complex member 1 L homeolog
  • babam1
  • BABAM1
  • babam1.L
  • Babam1
Epitope
Middle Region
11
10
7
4
3
2
2
1
1
1
1
Reactivity
Human
36
5
4
2
2
2
1
1
Host
Rabbit
29
4
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
34
20
19
6
4
3
1
Options
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN453692
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN652514 FACS IHC (p) WB Rabbit Ig Fraction AA 116-143, Center Log in to see Polyclonal 1
10 ABIN6749759 IHC IHC (p) WB Rabbit IgG AA 36-85 Log in to see Polyclonal 0
10 ABIN5536292 FACS IHC (p) WB Rabbit Ig Fraction AA 116-143 Log in to see Polyclonal 0
7 ABIN652513 IHC (p) WB Rabbit Ig Fraction AA 9-37, N-Term Log in to see Polyclonal 0
1 ABIN1812019 FACS IHC IHC (p) WB Rabbit AA 116-143 Log in to see Polyclonal 0
1 ABIN1812020 IHC IHC (p) WB Rabbit AA 9-37 Log in to see Polyclonal 0

General

Antigen BRISC and BRCA1 A Complex Member 1 (BABAM1) Antibodies
Epitope Middle Region
(11), (10), (7), (4), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(36), (5), (4), (2), (2), (2), (1), (1)
Host Rabbit
(29), (4), (2), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(34), (20), (19), (6), (4), (3), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody recognizes MERIT40/HSPC142 (Center).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen KLH conjugated synthetic peptide selected from the Center region of Human HSPC142

Target Details

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Antigen
Alternative Name MERIT40 (BABAM1 Antibody Abstract)
Background Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.Synonyms: BRCA1-A complex subunit MERIT40, C19orf62, HSPC142, Mediator of RAP80 interactions and targeting subunit of 40 kDa, NBA1, New component of the BRCA1-A complex
Gene ID 29086
NCBI Accession NP_001028721
UniProt Q9NWV8
Pathways Positive Regulation of Response to DNA Damage Stimulus

Application Details

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Application Notes ELISA: 1/1,000. Western blotting: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

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Background publications

Shao, Patterson-Fortin, Messick, Feng, Shanbhag, Wang, Greenberg: "MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks." in: Genes & development, Vol. 23, Issue 6, pp. 740-54, 2009

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (Middle Region) antibody (ABIN453692) Figure 2. Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antib...
Western Blotting (WB) image for anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (Middle Region) antibody (ABIN453692) Figure 1. Western blot analysis of HSPC142 Antibody (Center) (Cat#AP18061PU-N) in MDA...