anti-Human BRE antibody for Fluorescence Microscopy

Recommended BRE Antibody

Antigen
Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE) Antibodies
  • brcc4
  • brcc45
  • BRE
  • LOC100231620
  • BRCC4
  • BRCC45
  • 6030405P19Rik
  • AI429776
  • B830038C02Rik
  • im:7141046
  • zgc:110368
  • BRISC and BRCA1 A complex member 2
  • brain and reproductive organ-expressed (TNFRSF1A modulator)
  • BRISC and BRCA1 A complex member 2 L homeolog
  • babam2
  • BABAM2
  • Babam2
  • BRE
  • babam2.L
Epitope
N-Term
6
5
5
3
2
2
2
1
1
1
Reactivity
Human
71
49
41
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
68
3
Clonality
Polyclonal
Conjugate
This BRE antibody is un-conjugated
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
50
23
23
19
17
13
10
4
3
2
1
1
Options

Available images

Catalog No. ABIN6656869
$ 394.90
Plus shipping costs $45.00 and $22.60 dry ice

General

Antigen Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE) Antibodies
Epitope N-Term
(6), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human
(71), (49), (41), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(68), (3)
Clonality
Polyclonal
Conjugate This BRE antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
(50), (23), (23), (19), (17), (13), (10), (4), (3), (2), (1), (1)
Supplier

Product Details anti-BRE Antibody

Target Details BRE Application Details Handling Images
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Cross reactivity with BRCC45 from other sources has not been determined.
Purification Anti-BRCC45 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Immunogen

Immunogen: Anti-BRCC45 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the N-terminus of human BRCC45.

Immunogen Type: Peptide

Isotype IgG
Plasmids, Primers & others

Target Details BRE

Product Details anti-BRE Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRCC45 (BRE Antibody Abstract)
Background

Synonyms: BRCC45 Antibody, BRCC4, BRCC45, BRCA1-A complex subunit BRE, Brain and reproductive organ-expressed protein

Background: BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.

Gene Name: BRE

Gene ID 9577
NCBI Accession NP_004890
UniProt Q9NXR7
Pathways Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-BRE Antibody Target Details BRE Handling Images back to top
Application Notes

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-BRCC45 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 44 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions For Research Use only

Handling

Product Details anti-BRE Antibody Target Details BRE Application Details Images back to top
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Images

Product Details anti-BRE Antibody Target Details BRE Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE) (N-Term) antibody (ABIN6656869) Immunofluorescence of BRCC45 Antibody Immunofluorescence Microscopy of BRCC45 antibod...
Western Blotting (WB) image for anti-Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE) (N-Term) antibody (ABIN6656869) Western Blot of BRCC45 Antibody Western Blot of BRCC45 antibody. Lane A HeLa cell lys...
Immunohistochemistry (IHC) image for anti-Brain and Reproductive Organ-Expressed (TNFRSF1A Modulator) (BRE) (N-Term) antibody (ABIN6656869) Immunohistochemistry of BRCC45 Antibody Immunohistochemistry of BRCC45 antibody. Tiss...