anti-Human Tumor Necrosis Factor antibody for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Human, Non-Human Primate, Pig (Porcine)
1274
462
357
144
136
119
118
58
53
41
29
17
14
12
9
7
6
4
4
3
3
2
1
1
1
1
Host
Mouse
945
530
178
48
10
9
8
7
5
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to PE
171
97
72
51
49
33
31
28
28
26
26
26
26
25
25
25
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
876
856
621
327
317
229
224
184
137
105
96
67
29
28
26
25
20
18
14
11
9
9
7
6
6
5
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN1690740
$ 366.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.592816 ABIN2749165 Func IHC (f) ICC FACS ELISA APC Mouse IgG1 Log in to see MAb11 4
13.592816 ABIN4898910 FACS PE Mouse IgG1 Log in to see 6402 8
13.592816 ABIN2662374 FACS Pacific Blue Mouse IgG1 kappa Log in to see MAb11 11
13.592816 ABIN2662036 FACS FITC Mouse IgG1 kappa Log in to see MAb11 11
13.592816 ABIN2658839 FACS APC Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN4898909 FACS Fluorescein Mouse IgG1 Log in to see 6402 4
13.592816 ABIN2659610 FACS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2657286 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2657587 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2660503 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2660627 FACS APC-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN2663940 FACS PE Mouse IgG1 kappa Log in to see MAb11 10
13.592816 ABIN1997163 CC ELISA FACS Mouse IgG1 AA 77-233 Log in to see 9A7A5G9 1
13.592816 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
13.592816 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
13.592816 ABIN1997166 FACS FITC Mouse IgG1 AA 77-233 Log in to see 01 0
13.592816 ABIN1997168 FACS FITC Rabbit IgG AA 77-233 Log in to see 101 0
13.592816 ABIN1383957 FACS PE Mouse IgG1 Log in to see B-D9 1
13.592816 ABIN5503748 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Non-Human Primate, Pig (Porcine)
(1274), (462), (357), (144), (136), (119), (118), (58), (53), (41), (29), (17), (14), (12), (9), (7), (6), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(945), (530), (178), (48), (10), (9), (8), (7), (5), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to PE
(171), (97), (72), (51), (49), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(876), (856), (621), (327), (317), (229), (224), (184), (137), (105), (96), (67), (29), (28), (26), (25), (20), (18), (14), (11), (9), (9), (7), (6), (6), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1690740) Images
Specificity The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Recombinant human TNF-alpha
Clone MAb11
Isotype IgG1

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1690740) Images back to top
Antigen
Alternative Name TNF (TNF Antibody Abstract)
Background TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN1690740) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN1690740) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-Tumor Necrosis Factor antibody (ABIN1690740)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Cesaro-Tadic, Dernick, Juncker, Buurman, Kropshofer, Michel, Fattinger, Delamarche: "High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems." in: Lab on a chip, Vol. 4, Issue 6, pp. 563-9, 2004

Yan, Qing, Byers, Stadnyk, Al-Hertani, Bortolussi: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." in: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, 2004

Attarbaschi, Willheim, Ramharter, Hofmann, Wahl, Winkler, Graninger, Winkler: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." in: European cytokine network, Vol. 14, Issue 1, pp. 34-9, 2003

Visser, Graffelman, Blauw, Haspels, Lentjes, de Kloet, Nagelkerken: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." in: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, 2001

Wahlström, Katchar, Wigzell, Olerup, Eklund, Grunewald: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." in: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, 2001

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1690740) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (TNF) antibody (PE) (ABIN1690740) Intracellular staining of activated human peripheral blood with anti-TNF alpha (MAb11...