anti-Human Tumor Necrosis Factor antibody for Immunohistochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
1273
458
355
144
136
119
118
62
54
41
29
17
13
12
9
7
6
4
4
4
3
2
1
1
1
1
Host
Rabbit
948
528
177
50
10
9
8
7
5
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
172
96
73
51
49
33
31
28
28
26
26
26
26
25
25
25
24
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
915
864
621
323
288
230
224
190
143
107
97
67
29
29
26
22
20
18
14
11
9
7
6
6
6
6
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN153474
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.067091 ABIN2707185 IHC WB Rabbit Center Log in to see Polyclonal 0
9.067091 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 5
9.067091 ABIN1533583 IF IHC ELISA Rabbit IgG AA 141-190 Log in to see Polyclonal 0
9.067091 ABIN1875123 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
9.067091 ABIN1043796 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.067091 ABIN3188057 IHC WB Mouse IgG1 Log in to see Q36 0
9.067091 ABIN3188056 IHC WB Mouse IgG1 Log in to see Q34 0
9.067091 ABIN3188055 IHC WB Rabbit IgG Log in to see Polyclonal 0
9.067091 ABIN1172395 ICC IHC WB Rabbit IgG AA 77-233 Log in to see Polyclonal 0
9.067091 ABIN2852671 IHC WB Mouse Log in to see 0
9.067091 ABIN3020863 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
9.067091 ABIN2470862 IHC ELISA Mouse IgG1 kappa Log in to see MAb1 0
9.067091 ABIN107946 IHC Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.067091 ABIN3023991 FACS IF IHC Mouse IgG1, kappa AA 115-130 Log in to see SPM543 0
9.067091 ABIN2471395 IHC ELISA WB Mouse IgG1 kappa AA 1-234 Log in to see M 1 C4 0
9.067091 ABIN1846088 IF/ICC IHC Rabbit Log in to see Polyclonal 0
9.067091 ABIN2428843 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
9.067091 ABIN5596796 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
9.067091 ABIN1849100 ELISA IF/ICC IHC Rabbit Internal Region Log in to see Polyclonal 0
9.067091 ABIN5596795 ELISA IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(1273), (458), (355), (144), (136), (119), (118), (62), (54), (41), (29), (17), (13), (12), (9), (7), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(948), (528), (177), (50), (10), (9), (8), (7), (5), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(172), (96), (73), (51), (49), (33), (31), (28), (28), (26), (26), (26), (26), (25), (25), (25), (24), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(915), (864), (621), (323), (288), (230), (224), (190), (143), (107), (97), (67), (29), (29), (26), (22), (20), (18), (14), (11), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN153474) Images
Specificity The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNG beta (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Purification Delipidation and Defibrination
Immunogen Recombinant full length protein produced in E. coli (Human).

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN153474) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN153474) Images back to top
Application Notes Western Blot 1:500 - 1:2000, ELISA 1:1000 - 1:5000, Immunohistochemistry 1:100 - 1:500, Immunohistochemistry-Paraffin 1:100 - 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN153474) Images back to top
Format Liquid
Concentration 75 mg/mL
Buffer None
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Tumor Necrosis Factor antibody (ABIN153474)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Al-Khalaf, Amir, Al-Mohanna, Tulbah, Al-Sayed, Aboussekhra: "Obesity and p16(INK4A) Downregulation Activate Breast Adipocytes and Promote Their Protumorigenicity." in: Molecular and cellular biology, Vol. 37, Issue 17, 2017 (Sample species: Human).

Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Chen, Jian, Lien, Lin, Ting, Chen, Hsu, Huang, Wu, Kuan, Chao, Wu, Huang, Juan: "Adipocytes play an etiological role in the podocytopathy of high-fat diet-fed rats." in: The Journal of endocrinology, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting

Günel, Demirci, Eryılmaz, Yılmaz, Meteoğlu, Ömürlü, Başal: "Inhibitory Effect of Pycnogenol(®) on Airway Inflammation in Ovalbumin-Induced Allergic Rhinitis." in: Balkan medical journal, Vol. 33, Issue 6, pp. 620-626, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rat (Rattus)).

Chen, Zhang, Wang, Lei, Su, Zeng, Pei, Huang: "Inhibition of immunoproteasome reduces infarction volume and attenuates inflammatory reaction in a rat model of ischemic stroke." in: Cell death & disease, Vol. 6, pp. e1626, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Tessier, Raha, Holloway, Yockell-Lelièvre, Tayade, Gruslin: "Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation." in: Journal of reproductive immunology, Vol. 110, pp. 89-101, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Wang, Parrish: "Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells." in: Apoptosis : an international journal on programmed cell death, Vol. 20, Issue 7, pp. 921-9, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Rodriguez, Edrey, Osmulski, Gaczynska, Buffenstein: "Altered composition of liver proteasome assemblies contributes to enhanced proteasome activity in the exceptionally long-lived naked mole-rat." in: PLoS ONE, Vol. 7, Issue 5, pp. e35890, 2012 Further details: Western Blotting

Roth, Städler, Lemann, Hösli, Simon, Simon: "Distinct eosinophil cytokine expression patterns in skin diseases - the possible existence of functionally different eosinophil subpopulations." in: Allergy, Vol. 66, Issue 11, pp. 1477-86, 2011 (Sample species: Human). Further details: Immunohistochemistry

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN153474) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN153474) anti-Tumor Necrosis Factor (TNF) antibody
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN153474) Western Blot: TNF-alpha Antibody [ABIN153474] - Analysis using Azide/BSA FREE version...
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN153474) Immunohistochemistry: TNF-alpha Antibody [ABIN153474] - Staining of formalin/PFA-fixe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor (TNF) antibody (ABIN153474) Immunohistochemistry-Paraffin: TNF-alpha Antibody [ABIN153474] - Analysis using Azide...