anti-Rat (Rattus) NUP153 antibody for Western Blotting

Recommended NUP153 Antibody (supplied by: Log in to see )

Antigen
Nucleoporin 153kDa (NUP153) Antibodies
  • CG4453
  • Dmel\\CG4453
  • NUP153
  • Nup 153
  • dNup153
  • dmNup153
  • nup153
  • NUCZINK
  • B130015D15Rik
  • C88147
  • HNUP153
  • N153
  • Nucleoporin 153kD
  • nucleoporin 153
  • nucleoporin 153kDa L homeolog
  • Nup153
  • NUP153
  • nup153.L
Epitope
AA 610-1191
8
5
4
3
3
2
2
1
1
1
Reactivity
Rat (Rattus)
42
12
7
3
2
2
1
1
1
1
1
1
1
Host
Rat
39
5
1
1
Clonality
Monoclonal
Conjugate
This NUP153 antibody is un-conjugated
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
36
15
15
12
10
8
8
6
3
1
1
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Catalog No. ABIN2452062
$ 494.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.08196 ABIN2788808 WB Rabbit C-Term Log in to see Polyclonal 0
3.08196 ABIN5584850 ELISA ICC IF WB Rat IgG2b kappa Log in to see R4C8 0
1 ABIN341029 IF IP WB Sheep AA 1456-1475 Log in to see Polyclonal 0
1 ABIN126026 IHC (fro) IF IP WB Mouse IgG1 Log in to see nup7A8 4
1 ABIN6263773 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN481543 IF IHC IHC (fro) IP WB Mouse IgG1 Log in to see 0
1 ABIN2917942 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1089637 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN284664 IHC (fro) IF IP WB Mouse IgG1 Log in to see nup7A8 0
1 ABIN4956499 IHC IP WB Mouse IgG1 Log in to see nup7A8 0
1 ABIN5873233 ELISA IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Nucleoporin 153kDa (NUP153) Antibodies
Epitope AA 610-1191
(8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Reactivity Rat (Rattus)
(42), (12), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rat
(39), (5), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NUP153 antibody is un-conjugated
(2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Western Blotting (WB)
(36), (15), (15), (12), (10), (8), (8), (6), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-NUP153 Antibody

Target Details NUP153 Application Details Handling References for anti-NUP153 antibody (ABIN2452062) Images
Specificity Specific to human, mouse, rat and monkey Nup153 proteins.
Cross-Reactivity (Details) Not tested for other species
Characteristics When injected into the nucleus,R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.
Purification Purified
Sterility Sterile filtered
Immunogen Recombinant GST-fused rat Nup153 (610-1191aa, Zn finger and FG repeats domain)
Clone R4C8
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details NUP153

Product Details anti-NUP153 Antibody Application Details Handling References for anti-NUP153 antibody (ABIN2452062) Images back to top
Antigen
Alternative Name Nup153 (NUP153 Antibody Abstract)
Background The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153 kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
UniProt P49790
Pathways Protein targeting to Nucleus

Application Details

Product Details anti-NUP153 Antibody Target Details NUP153 Handling References for anti-NUP153 antibody (ABIN2452062) Images back to top
Application Notes 1. Western blotting: 160 kDa single band in Hela cell extract
2. Immunocytochemistry
3. ELISA
Other applications have not been tested.

Additional comments:
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
R4C8 works in immuno-cytochemistry very well.
Restrictions For Research Use only

Handling

Product Details anti-NUP153 Antibody Target Details NUP153 Application Details References for anti-NUP153 antibody (ABIN2452062) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol
Preservative Azide free
Storage -20 °C
Storage Comment Upon receipt aliquot and store at -20 C

References for anti-NUP153 antibody (ABIN2452062)

Product Details anti-NUP153 Antibody Target Details NUP153 Application Details Handling Images back to top
Product cited in:

Lim, Fahrenkrog, Köser, Schwarz-Herion, Deng, Aebi: "Nanomechanical basis of selective gating by the nuclear pore complex." in: Science (New York, N.Y.), Vol. 318, Issue 5850, pp. 640-3, 2007

Ullman, Shah, Powers, Forbes: "The nucleoporin nup153 plays a critical role in multiple types of nuclear export." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 649-64, 1999

Images

Product Details anti-NUP153 Antibody Target Details NUP153 Application Details Handling References for anti-NUP153 antibody (ABIN2452062) back to top
Supplier Images
Western Blotting (WB) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody
Immunofluorescence (IF) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 2)
Immunofluorescence (IF) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 3)
Western Blotting (WB) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 4)
Immunofluorescence (IF) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 5)
Immunofluorescence (IF) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 6)
Immunofluorescence (IF) image for anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (ABIN2452062) anti-Nucleoporin 153kDa (NUP153) (AA 610-1191) antibody (Image 7)