Neurturin (NRTN) Protein

Details for Product No. ABIN1004936
Protein Name
Origin
Human
9
1
Source
Escherichia coli (E. coli)
8
2
Protein Type
Recombinant
Options
Characteristics Recombinant Human Neurturin (NTN). Human Neurturin at a concentration of 100 ng/mL will support the survival of 65% of newborn rat sympathetic neurons.
Purity > 98 % (determined by SDS-PAGE and HPLC analysis).
Sterility Sterile filtered
Endotoxin Level < 0.1 ng per µg (1EU/µg) of rHu NTN.
Protein Name
Background Neurturin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Neurturin signals through a multicomponent receptor system, composed of RET and one of four GFR-alpha (alpha1-alpha4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRalpha2. The functional form of human Neurturin is a disulfide-linked homodimer, of two 11.8 kDa polypeptide monomers (206 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Molecular Weight 23.6 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Buffer Lyophilized from a sterile-filtered solution in 10 mM sodium citrate (pH 4).
Storage -20 °C
Storage Comment The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted NTN should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.