Inhibin, beta A (INHBA) (Active) protein (His tag)

Details for Product No. ABIN1019683, Supplier: Log in to see
Protein Name
  • actba
  • inhba
  • inhbaa-001
  • edf
  • frp
  • EDF
  • FRP
  • INHB
  • inhibin, beta Aa
  • inhibin beta A subunit
  • inhibin beta A
  • inhibin beta-A
  • inhbaa
  • INHBA
  • inhba
  • Inhba
Origin
Human
23
5
4
3
3
3
2
2
1
Source
Tobacco (Nicotiana benthamiana)
20
6
4
4
3
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This INHBA protein is labelled with His tag.
Application
Cell Culture (CC), Western Blotting (WB)
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Sequence HHHHHHGLEC DGKVNICCKK QFFVSFKDIG WNDWIIAPSG YHANYCEGEC PSHIAGTSGS SLSFHSTVIN HYRMRGHSPF ANLKSCCVPT KLRPMSMLYY DDGQNIIKKD IQNMIVEECG CS
Specificity Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin A.
Characteristics Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin A.
Molecular formula: C600H911N173O174S13
Isoelectric Point: 7,27.
Extinction coefficient: E 0.1 % (1g/L) = 1.27 (A 280 nm).
This product contains no animal-derived components or impurities. Produced by transient expression of Activin A in non-transgenic plants.
Purification Recombinant human Activin A contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).
Purity > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Endotoxin Level < 0.04 EU/µg protein (LAL method)
Protein Name
Background Synonyms: Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein
Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGF-beta family. Mature Activin A has two 116 amino acids residues betaA subunits (betaA-betaA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
Molecular Weight 27.4 kDa
Gene ID 3624, 147290
UniProt P08476
Pathways Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagy
Application Notes Ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation)
Comment

Biological Activity: The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation). ED50 ≤ 5 ng/ml.
Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Activin A contains a 6-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Lyophilized protein should be reconstituted in water to a concentration of 50 ng/µL. Due to the protein nature, dimmers and multimers may be observed. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.
Concentration 50 ng/µL
Buffer Tris HCl 0.05 M buffer at pH 7.4.
Storage 4 °C
Supplier Images
 image for Inhibin, beta A (INHBA) (Active) protein (His tag) (ABIN1019683) SDS-PAGE analysis of recombinant Activin A. Samples were loaded in 15 % SDS-polyacryl...
 image for Inhibin, beta A (INHBA) (Active) protein (His tag) (ABIN1019683) Western Blot analysis of recombinant Activin A. Lane MWM: Molecular weight marker (kD...
 image for Inhibin, beta A (INHBA) (Active) protein (His tag) (ABIN1019683) The biological activity of Activin A is measured by its ability to inhibit mouse plas...
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