IL12B Protein (AA 23-328) (His tag)

Catalog No. ABIN1019696

Product Details

Target: IL12B (Interleukin 12 beta (IL12B))

Protein Type: Recombinant

Protein Characteristics: AA 23-328

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Purification tag / Conjugate: This IL12B protein is labelled with His tag.

Application: Western Blotting (WB), Immunogen (Imm)

Sequence: HHHHHHIWEL KKDVYVVELD WYPDAPGEMV VLTCDTPEED GITWTLDQSS EVLGSGKTLT IQVKEFGDAG QYTCHKGGEV LSHSLLLLHK KEDGIWSTDI LKDQKEPKNK TFLRCEAKNY SGRFTCWWLT TISTDLTFSV KSSRGSSDPQ GVTCGAATLS AERVRGDNKE YEYSVECQED SACPAAEESL PIEVMVDAVH KLKYENYTSS FFIRDIIKPD PPKNLQLKPL KNSRQVEVSW EYPDTWSTPH SYFSLTFCVQ VQGKSKREKK DRVFTDKTSA TVICRKNASI SVRAQDRYYS SSWSEWASVP CS

Specificity: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific IL12 antibody.

Characteristics: Molecular Formula: C1575H2420N424O490S12
Isoelectric Point: 5,91
Extinction Coefficient: E 0.1 % (1g/L) = 2.069 (A 280 nm)
This product contains no animal-derived components or impurities. Produced by transient expression of Interleukin-12 subunit p40 in non-transgenic plants.

Purification: Recombinant human Interleukin-12 subunit p40 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Purity: > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

Biological Activity: The biological activity of IL12 p40 is determined by the dose dependent inhibition of IL-12 dependent IFN-gamma production (mouse splenocytes). No tested
Recombinant human Interleukin-12 p40 is a glycosylated polypeptide chain containing 312 amino acids ( fragment 23 - 328 P29460 IL12B_HUMAN) with an amino- terminal hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDSPAGE under reducing conditions. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Interleukin-12 subunit p40 contains a 6-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 50 ng/µL. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.

Buffer: 20 mM PBS buffer at pH 7.4

Storage: 4 °C

Target Details

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B (IL12B Products)

Synonyms: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, IL-12 p40 Protein, il12b Protein, il12p40.b Protein, zgc:152789 Protein, IL-12.p40 Protein, IL12p40 Protein, IL12B Protein, CLMF p40 Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12Ba Protein, IL12B Protein, Il12b Protein, il12ba Protein

Background: Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-gamma, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. The p40 subunit of IL-12 interacts with the beta subunit of IL-12R and the disulfide linked p40 homodimer specifically antagonize the activity of bioactive IL-12 in different assay systems.

Molecular Weight: 35.5 kDa

Gene ID: 3593, 161561

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation