RANKL Protein (AA 70-244)

Catalog No. ABIN1019704

Product Details

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Protein Type: Recombinant

Protein Characteristics: AA 70-244

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Application: Western Blotting (WB), Immunogen (Imm)

Sequence: HHHHHHHHHH EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDID

Specificity: Serological Identification: The protein was analysed by Dot-blot with specifics antibodies

Characteristics: Molecular Formula: C952H1414N262O276S5
Isoelectric Point: 6.82
Extinction Coefficient: E 0.1 % (1g/L) = 1.678 (A 280 nm)

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

rhuman sRANKL is a glycosylated polypeptide chain containing 175 amino acids (70 - 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosylation, the recombinant protein could migrate as two bands with an apparent molecular mass of 21-23 kDa in SDSPAGE. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Receptor activator of nuclear factor kappa-B ligand (sRANK-ligand) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 200 ng/µL. Optimal concentration should be determined for specific application and cell lines.

Buffer: 10 mM Phosphate Potasium buffer pH 8 and 0.2 M NaCl

Storage: 4 °C

Target Details

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Abstract: TNFSF11 Products

Synonyms: CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, TNFSF11 Protein, Ly109l Protein, OPG Protein, Trance Protein, cd254 Protein, odf Protein, opgl Protein, optb2 Protein, rankl Protein, sodf Protein, tnfsf11 Protein, trance Protein, TNF superfamily member 11 Protein, tumor necrosis factor (ligand) superfamily, member 11 Protein, TNF receptor superfamily member 11b Protein, TNF superfamily member 11 L homeolog Protein, TNFSF11 Protein, Tnfsf11 Protein, TNFRSF11B Protein, tnfsf11.L Protein

Background: Synonyms: Tumor necrosis factor ligand superfamily member 11 (TNFSF11), Osteoprotegerin ligand (OPGL), TNF-related activation-induced cytokine (TRANCE)
Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodelling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signalling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases.

Molecular Weight: 21.1 kDa

UniProt: O14788

Pathways: NF-kappaB Signaling