Follistatin Protein (FST) (AA 30-217)

Catalog No. ABIN1019719

Product Details

Target: Follistatin (FST)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 30-217

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Application: Western Blotting (WB), Cell Culture (CC)

Sequence: HHHHHHGNCW LRQAKNGRCQ VLYKTELSKE ECCSTGRLST SWTEEDVNDN TLFKWMIFNG GAPNCIPCKE TCENVDCGPG KKCRMNKKNK PRCVCAPDCS NITWKGPVCG LDGKTYRNEC ALLKARCKEQ PELEVQYQGR CKKTCRDVFC PGSSTCVVDQ TNNAYCVTCN RICPEPASSE QYLCGNDGVT YSSACHLRKA TCLLGRSIGL AYEGKCIKAK SCEDIQCTGG KKCLWDFKVG RGRCSLCDEL CPDSKSDEPV CASDNATYAS ECAMKEAACS SGVLLEVKHS GSCN

Specificity: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific Follistatin antibody.

Characteristics: Molecular Formula: C1350H2153N405O433S39
Isoelectric Point: 8.18
Extinction Coefficient: 1.28

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Comment:

rhuman Follistatin isoform 2 is a polypeptide chain containing 217 amino acids (30-217 of P19883 FST_HUMAN). It has a predicted molecular mass of 32.3 kDa. Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal Free product. The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with coomassie blue.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 50 ng/µL. Optimal concentration should be determined for specific application and cell lines.

Buffer: Tris HCl 0.05M buffer at pH 7.4

Storage: 4 °C

Target Details

Target: Follistatin (FST)

Alternative Name: Follistatin (FST Products)

Synonyms: CG12955 Protein, CG12956 Protein, CG33466 Protein, Dmel\\CG33466 Protein, Fol1 Protein, dFS Protein, dFol1 Protein, dfs Protein, fs Protein, FS Protein, foll Protein, xfs Protein, FST Protein, fst Protein, AL033346 Protein, FOL1 Protein, Fst-288 Protein, RATFOL1 Protein, cb446 Protein, fd07h10 Protein, fst1 Protein, wu:fd07h10 Protein, Follistatin Protein, follistatin Protein, follistatin L homeolog Protein, follistatin a Protein, Fs Protein, fst Protein, FST Protein, LOC100141662 Protein, fst.L Protein, Fst Protein, fsta Protein

Background: Synonyms: FS288, Activin-binding protein
Follistatin is a monomeric glycoprotein that binds to ligands of the TGF-beta superfamily and regulates their activity by inhibiting their access to signalling receptors. Follistatin received its name because it suppresses synthesis and secretion of follicle-stimulating hormone (FSH) from the pituitary gland. The follistatin gene localizes to chromosome 5q11.2. It is composed of a relatively small 6-kb genomic DNA consisting of six exons. There is an alternative splice site that generates two major species, a full-length version that encodes a 344-amino acid preprotein differing by a 27-amino acid sequence from its carboxy-shortened version of the 317-amino acid form missing exon 6. Prior to activation, follistatin, like myostatin, undergoes further posttranslational modification to lose another 29 amino acids by removal of the signal peptide that results in polypeptides of 315 (FS315), often referred to as the long isoform and 288 (FS288), called the short isoform.

Molecular Weight: 32.3 kDa

Pathways: Negative Regulation of Hormone Secretion