DDX39B Protein (AA 2-251, partial) (GST tag)

Catalog No. ABIN1047670

Product Details

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Protein Type: Recombinant

Protein Characteristics: AA 2-251, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DDX39B protein is labelled with GST tag.

Application: ELISA, SDS-PAGE (SDS)

Sequence: AENDVDNELL DYEDDEVETA AGGDGAEAPA KKDVKGSYVS IHSSGFRDFL LKPELLRAIV DCGFEHPSEV QHECIPQAIL GMDVLCQAKS GMGKTAVFVL ATLQQLEPVT GQVSVLVMCH TRELAFQISK EYERFSKYMP NVKVAVFFGG LSIKKDEEVL KKNCPHIVVG TPGRILALAR NKSLNLKHIK HFILDECDKM LEQLDMRRDV QEIFRMTPHE KQVMMFSATL SKEIRPVCRK FMQDPMEIFV

Characteristics: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Ref.9 Ref.10 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.24 Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4. Ref.9 Ref.10 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.24

Purity: 90 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS buffer, 20mM GSH

Storage: -20 °C

References

Peelman, Chardon, Nunes, Renard, Geffrotin, Vaiman, Van Zeveren, Coppieters, van de Weghe, Bouquet: "The BAT1 gene in the MHC encodes an evolutionarily conserved putative nuclear RNA helicase of the DEAD family." in: Genomics, Vol. 26, Issue 2, pp. 210-8, (1995) (PubMed).

Target Details

Target: DDX39B (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 39B (DDX39B))

Alternative Name: Spliceosome RNA helicase DDX39B (DDX39B Products)

Synonyms: BAT1 Protein, D6S81E Protein, UAP56 Protein, Bat1 Protein, Bat1a Protein, p47 Protein, 0610030D10Rik Protein, AI428441 Protein, Bat-1 Protein, D17H6S81E Protein, D17H6S81E-1 Protein, D6S81Eh Protein, bat1 Protein, zgc:63773 Protein, DExD-box helicase 39B Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B Protein, DDX39B Protein, Ddx39b Protein, ddx39b Protein

Background: Synonyms: 56 kDa U2AF65-associated protein,ATP-dependent RNA helicase p47,DEAD box protein UAP56,HLA-B-associated transcript 1 protein

Molecular Weight: 55.6 kD

UniProt: Q13838

Pathways: Ribonucleoprotein Complex Subunit Organization