Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Annexin V Protein (Alexa Fluor 488)

ANXA5 Origin: Mouse Recombinant
Catalog No. ABIN1047952
  • Target See all Annexin V (ANXA5) Proteins
    Annexin V (ANXA5) (Annexin A5 (ANXA5))
    Protein Type
    Recombinant
    Origin
    • 16
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    Please inquire
    Purification tag / Conjugate
    This Annexin V protein is labelled with Alexa Fluor 488.
    Characteristics
    Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
    Gene Name: Anxa5
    Material not included
    Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
    Propidium iodide: 50 g/ml, final concentration 1 g/ml
    Top Product
    Discover our top product ANXA5 Protein
  • Application Notes
    5 l are suitable for one assay
    Assay Procedure

    Staining protocol:
    1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
    2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
    For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
    3. Incubate for 15min at room temperature in the dark
    4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).

    Restrictions
    For Research Use only
  • Format
    Liquid
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Vermes, Haanen, Steffens-Nakken, Reutelingsperger: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).

  • Target
    Annexin V (ANXA5) (Annexin A5 (ANXA5))
    Alternative Name
    Annexin V-Alexa488 (ANXA5 Products)
    Synonyms
    anx Protein, anx5 Protein, ANX V Protein, anxa5 Protein, cb989 Protein, wu:fa98f06 Protein, wu:fj10f10 Protein, MGC89158 Protein, ANX5 Protein, ENX2 Protein, PP4 Protein, RPRGL3 Protein, Anx5 Protein, R74653 Protein, LC5 Protein, enx2 Protein, annexin A5 Protein, annexin A5b Protein, Annexin A5 Protein, annexin A5 L homeolog Protein, ANXA5 Protein, anxa5b Protein, anxa5 Protein, Anxa5 Protein, anxa5.L Protein
    Gene ID
    11747
    UniProt
    P48036
    Pathways
    Apoptosis
You are here:
Support