AKR1C4 Protein (AA 1-323) (His tag)
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- Target See all AKR1C4 Proteins
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-323
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AKR1C4 protein is labelled with His tag.
- Purpose
- Recombinant Human Aldo-Keto Reductase 1C4/AKR1C4 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNY RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
- Characteristics
- Recombinant Human Aldo-Keto Reductase 1C4/AKR1C4 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Tyr323) of Human AKR1C4 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product AKR1C4 Protein
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Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-343) protein (His tag)
AKR1C4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (GST tag)AKR1C4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (Strep Tag)Crystallography grade AKR1C4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) (Active) proteinAKR1C4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) protein (Myc-DYKDDDDK Tag)AKR1C4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
- Alternative Name
- Aldo-Keto Reductase 1C4/AKR1C4 (AKR1C4 Products)
- Synonyms
- 3-alpha-HSD Protein, C11 Protein, CDR Protein, CHDR Protein, DD-4 Protein, DD4 Protein, HAKRA Protein, aldo-keto reductase family 1 member C4 Protein, AKR1C4 Protein
- Sub Type
- Fusionprotein
- Background
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Aldo-Keto Reductase 1C4/AKR1C4 is a member of the aldo/keto reductase family that consists of more than 40 known enzymes and proteins. AKR1C4 has highly expressed in Liver. It can catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. AKR1C4 catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-Androstan-3-alpha,17-beta-diol (3-alpha-diol). In addition, AKR1C4 also has some 20-alpha-Hydroxysteroid Dehydrogenase activity.
Alternative Names: Aldo-Keto Reductase Family 1 Member C4, 3-Alpha-HSD1, 3-Alpha-Hydroxysteroid Dehydrogenase Type I, Chlordecone Reductase, CDR, Dihydrodiol Dehydrogenase 4, DD-4, DD4, HAKRA, AKR1C4, CHDR - Molecular Weight
- 39.3 kDa
- UniProt
- P17516
- Pathways
- Steroid Hormone Biosynthesis
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