Alkaline Phosphatase Protein (ALP) (AA 18-502) (His tag)

Catalog No. ABIN1095988

Product Details

Target: Alkaline Phosphatase (ALP)

Protein Type: Recombinant

Protein Characteristics: AA 18-502

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This Alkaline Phosphatase protein is labelled with His tag.

Purpose: Recombinant Human Alkaline Phosphatase/ALPL (C-6His)

Sequence: LVPEKEKDPK YWRDQAQETL KYALELQKLN TNVAKNVIMF LGDGMGVSTV TAARILKGQL HHNPGEETRL EMDKFPFVAL SKTYNTNAQV PDSAGTATAY LCGVKANEGT VGVSAATERS RCNTTQGNEV TSILRWAKDA GKSVGIVTTT RVNHATPSAA YAHSADRDWY SDNEMPPEAL SQGCKDIAYQ LMHNIRDIDV IMGGGRKYMY PKNKTDVEYE SDEKARGTRL DGLDLVDTWK SFKPRYKHSH FIWNRTELLT LDPHNVDYLL GLFEPGDMQY ELNRNNVTDP SLSEMVVVAI QILRKNPKGF FLLVEGGRID HGHHEGKAKQ ALHEAVEMDR AIGQAGSLTS SEDTLTVVTA DHSHVFTFGG YTPRGNSIFG LAPMLSDTDK KPFTAILYGN GPGYKVVGGE RENVSMVDYA HNNYQAQSAV PLRHETHGGE DVAVFSKGPM AHLLHGVHEQ NYVPHVMAYA ACIGANLGHC APASSVDHHH HHH

Characteristics: Recombinant Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme/ALPL is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Leu18-Ser502) of Human ALPL fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, 2 mM MgSO4, 0.1 mM ZnCl2, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase (ALP Products)

Synonyms: ALP Protein, ALP1 Protein, CRASH Protein, ECK0378 Protein, JW0374 Protein, psiA Protein, 2410004D18Rik Protein, AU040643 Protein, AW060375 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, Actn2lp Protein, Alp Protein, asparaginase like 1 Protein, bacterial alkaline phosphatase Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, PDZ and LIM domain 3 Protein, ASRGL1 Protein, phoA Protein, Asrgl1 Protein, CCL27 Protein, alpl Protein, alp Protein, Pdlim3 Protein

Sub Type: Fusionprotein

Background: Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP(PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia,characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.
Alternative Names: Alkaline Phosphatase, Tissue-Nonspecific Isozyme, AP-TNAP, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme, ALPL

Molecular Weight: 54.5 kDa

UniProt: P05186