AIF Protein (AA 121-613) (His tag)

Catalog No. ABIN1096041

Product Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Protein Type: Recombinant

Protein Characteristics: AA 121-613

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AIF protein is labelled with His tag.

Purpose: Recombinant Human Apoptosis-Inducing Factor 1 Mitochondrial/AIFM1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MEEVPQDKAP SHVPFLLIGG GTAAFAAARS IRARDPGARV LIVSEDPELP YMRPPLSKEL WFSDDPNVTK TLRFKQWNGK ERSIYFQPPS FYVSAQDLPH IENGGVAVLT GKKVVQLDVR DNMVKLNDGS QITYEKCLIA TGGTPRSLSA IDRAGAEVKS RTTLFRKIGD FRSLEKISRE VKSITIIGGG FLGSELACAL GRKARALGTE VIQLFPEKGN MGKILPEYLS NWTMEKVRRE GVKVMPNAIV QSVGVSSGKL LIKLKDGRKV ETDHIVAAVG LEPNVELAKT GGLEIDSDFG GFRVNAELQA RSNIWVAGDA ACFYDIKLGR RRVEHHDHAV VSGRLAGENM TGAAKPYWHQ SMFWSDLGPD VGYEAIGLVD SSLPTVGVFA KATAQDNPKS ATEQSGTGIR SESETESEAS EITIPPSTPA VPQAPVQGED YGKGVIFYLR DKVVVGIVLW NIFNRMPIAR KIIKDGEQHE DLNEVAKLFN IHED

Characteristics: Recombinant Human Apoptosis-Inducing Factor 1, Mitochondrial/AIFM1 is produced by our E. coli expression system. The target protein is expressed with sequence (Glu121-Asp613) of Human AIFM1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIF (AIFM1 Products)

Synonyms: AIF Protein, CMTX4 Protein, COWCK Protein, COXPD6 Protein, PDCD8 Protein, CG7263 Protein, DmAIF Protein, Dmel\\CG7263 Protein, GB16024 Protein, DDBDRAFT_0187853 Protein, DDBDRAFT_0191137 Protein, DDB_0187853 Protein, DDB_0191137 Protein, aif Protein, pdcd8 Protein, AIFM1 Protein, PCD8 Protein, AIFsh2 Protein, Hq Protein, Pdcd8 Protein, mAIF Protein, Aif Protein, zgc:91994 Protein, apoptosis inducing factor mitochondria associated 1 Protein, allograft inflammatory factor 1 Protein, Apoptosis inducing factor Protein, apoptosis-inducing factor 1, mitochondrial Protein, apoptosis inducing factor Protein, apoptosis inducing factor, mitochondria associated 1 Protein, apoptosis-inducing factor, mitochondrion-associated, 1 Protein, apoptosis-inducing factor, mitochondrion-associated 1 Protein, AIFM1 Protein, AIF1 Protein, AIF Protein, LOC412212 Protein, aif Protein, aifm1 Protein, Aifm1 Protein

Sub Type: Fusionprotein

Background: Apoptosis-Inducing Factor 1, Mitochondrial (AIFM1) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. During apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces parthanatos i.e., caspase-independent fragmentation of chromosomal DNA. AIFM1 interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. It binds to DNA in a sequence-independent manner and plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells.ReferencesTezel G, et al. Immunoproteomic analysis of potential serum biomarker candidates in human glaucoma.PMID: 23150628 [PubMed - indexed for MEDLINE] PMCID: PMC3522442 http://www.ncbi.nlm.nih.gov/pubmed/23150628
Alternative Names: Apoptosis-Inducing Factor 1 Mitochondrial, Programmed Cell Death Protein 8, AIFM1, AIF, PDCD8

Molecular Weight: 56.2 kDa

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect