LYPLA1 Protein (AA 1-230) (His tag)
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- Target See all LYPLA1 Proteins
- LYPLA1 (Lysophospholipase I (LYPLA1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-230
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LYPLA1 protein is labelled with His tag.
- Purpose
- Recombinant Human Acyl-Protein Thioesterase 1/APT-1 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MCGNNMSTPL PAIVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIRSSHIK YICPHAPVRP VTLNMNVAMP SWFDIIGLSP DSQEDESGIK QAAENIKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFPQGPIGGA NRDISILQCH GDCDPLVPLM FGSLTVEKLK TLVNPANVTF KTYEGMMHSS CQQEMMDVKQ FIDKLLPPID
- Characteristics
- Recombinant Human APT-1/LYPLA1 produced by E. coli expression system. Target protein is expressed with sequence (Met1-Asp230) of Human LYPLA1 (Uniprot #O75608) fused with a His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Lysophospholipase I (LYPLA1) (AA 1-230) protein (GST tag)
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Lysophospholipase I (LYPLA1) (AA 1-230) protein (Strep Tag)Crystallography grade LYPLA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Lysophospholipase I (LYPLA1) (AA 1-230) protein (Strep Tag)Crystallography grade LYPLA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Lysophospholipase I (LYPLA1) (AA 1-230), (full length) protein (GST tag)LYPLA1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Comment
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Gene Name: LYPLA1 APT1 LPL1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- LYPLA1 (Lysophospholipase I (LYPLA1))
- Alternative Name
- Acyl-Protein Thioesterase 1/APT-1 (LYPLA1 Products)
- Synonyms
- APT-1 Protein, APT1 Protein, LPL-I Protein, LPL1 Protein, hAPT1 Protein, Pla1a Protein, 25KDL Protein, zgc:110260 Protein, lysophospholipase I Protein, lysophospholipase 1 Protein, lysophospholipase I L homeolog Protein, LYPLA1 Protein, Lypla1 Protein, lypla1 Protein, lypla1.L Protein
- Molecular Weight
- 26.83 kDa
- UniProt
- O75608
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