BIN2 Protein (AA 1-244) (His tag)
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- Target See all BIN2 Proteins
- BIN2 (Bridging Integrator 2 (BIN2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-244
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BIN2 protein is labelled with His tag.
- Purpose
- Recombinant Human Bridging Integrator 2/BIN2/BRAP1 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF YQQQAEGHKL YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK AIVWNNDLLW EDYEEKLADQ AVRTMEIYVA QFSEIKERIA KRGRKLVDYD SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA QTVFEDLNQE LLEELPILYN SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK QHSN
- Characteristics
- Recombinant Human Bridging Integrator 2/BIN2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Asn244) of Human BIN2 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BIN2 Protein
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Bridging Integrator 2 (BIN2) (AA 1-565) protein (Strep Tag)
Crystallography grade BIN2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Bridging Integrator 2 (BIN2) (AA 1-489) protein (Strep Tag)Crystallography grade BIN2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Bridging Integrator 2 (BIN2) protein (Myc-DYKDDDDK Tag)BIN2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Bridging Integrator 2 (BIN2) (AA 1-597) protein (His tag)BIN2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 80 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- BIN2 (Bridging Integrator 2 (BIN2))
- Alternative Name
- BIN2 (BIN2 Products)
- Synonyms
- BRAP-1 Protein, BIN2 Protein, bin2l Protein, wu:fi05e01 Protein, zgc:55758 Protein, bridging integrator 2 Protein, bridging integrator 2b Protein, BIN2 Protein, Bin2 Protein, bin2b Protein
- Sub Type
- Fusionprotein
- Background
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Bridging Integrator 2 (BIN2) is a cytoplasmic protein. BIN2 contains one BAR domain and Interacts with BIN1. BIN2 is highly expressed in some hematopoietic tissues, including peripheral blood, thymus, colon and placenta. BIN2 is an Arabidopsis GSK3-like kinase that negatively regulates brassinosteroid (BR) signaling. Genetic studies show that BIN2 is inhibited in response to BR perception at the cell surface to relieve its inhibitory effects on downstream targets.
Alternative Names: Bridging Integrator 2, Breast Cancer-Associated Protein 1, BIN2, BRAP1 - Molecular Weight
- 30.58 kDa
- UniProt
- Q9UBW5
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