LSM1 Protein (AA 1-133) (His tag)

Catalog No. ABIN1096191

Product Details

Target: LSM1 (LSM1 Homolog, U6 Small Nuclear RNA Associated (LSM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-133

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LSM1 protein is labelled with His tag.

Purpose: Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm1/LSM1 (C-6His)

Sequence: MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEYVEHHHHH H

Characteristics: Recombinant Human U6 snRNA-Associated Sm-Like Protein LSm1/LSM1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Tyr133) of Human LSM1 fused with a His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: LSM1 (LSM1 Homolog, U6 Small Nuclear RNA Associated (LSM1))

Alternative Name: Cancer-associated-Sm-like (LSM1 Products)

Synonyms: lsm1 Protein, NV45 Protein, CASM Protein, YJL124C Protein, 2810025O06Rik Protein, zgc:101136 Protein, LSM1 homolog, mRNA degradation associated Protein, LSM1 homolog, U6 small nuclear RNA associated Protein, LSM1 homolog, mRNA degradation associated L homeolog Protein, LSM1, U6 small nuclear RNA associated Protein, lsm1 Protein, Lsm1 Protein, lsm1.L Protein, LSM1 Protein

Sub Type: Fusionprotein

Background: U6 snRNA-Associated Sm-Like Protein LSm1 (LSM1) belongs to the snRNP Sm proteins family. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which plays an important role in pre-mRNA splicing. LSM1 binds specifically to the 3-terminal U-tract of U6 snRNA. LSM1 can interact with SLBP when histone mRNA is being rapidly degraded during the S phase. In addition, LSM1 is associated with cellular transformation and the progression of several malignancies including mesothelioma, lung cancer and breast cancer.
Alternative Names: U6 snRNA-Associated Sm-Like Protein LSm1, Cancer-Associated Sm-Like, Small Nuclear Ribonuclear CaSm, LSM1, CASM

Molecular Weight: 16.23 kDa

UniProt: O15116