CPA1 Protein (AA 17-419) (His tag)

Catalog No. ABIN1096218

Product Details

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Protein Type: Recombinant

Protein Characteristics: AA 17-419

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CPA1 protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)

Sequence: KEDFVGHQVL RISVADEAQV QKVKELEDLE HLQLDFWRGP AHPGSPIDVR VPFPSIQAVK IFLESHGISY ETMIEDVQSL LDEEQEQMFA FRSRARSTDT FNYATYHTLE EIYDFLDLLV AENPHLVSKI QIGNTYEGRP IYVLKFSTGG SKRPAIWIDT GIHSREWVTQ ASGVWFAKKI TQDYGQDAAF TAILDTLDIF LEIVTNPDGF AFTHSTNRMW RKTRSHTAGS LCIGVDPNRN WDAGFGLSGA SSNPCSETYR GKFANSEVEV KSIVDFVKDH GNIKAFISIH SYSQLLMYPY GYKTEPVPDQ DELDQLSKAA VTALASLYGT KFNYGSIIKA IYQASGSTID WTYSQGIKYS FTFELRDTGR YGFLLPASQI IPTAKETWLA LLTIMEHTLN HPYVDHHHHH H

Characteristics:

Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: cpa1 (CPA1 Products)

Synonyms: cpa1 Protein, cbpa1 Protein, CPA Protein, CPA1 Protein, 0910001L12Rik Protein, Cpa Protein, carboxypeptidase A1 Protein, pancreatic carboxypeptidase A1 Protein, Carboxypeptidase A1 Protein, carboxypeptidase A1-like Protein, carboxypeptidase a1 Protein, carboxypeptidase A1, pancreatic Protein, CpipJ_CPIJ007169 Protein, CpipJ_CPIJ010802 Protein, CpipJ_CPIJ010805 Protein, MCYG_06789 Protein, MGYG_03840 Protein, cpa1 Protein, cbpa1 Protein, LOC100224866 Protein, CPA1 Protein, Cpa1 Protein

Background: Recombinant Human Carboxypeptidase A1/CPA1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala111-Tyr419) of Human CPA1 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.

Molecular Weight: 46.57 kDa

UniProt: P15085