CPB1 Protein (AA 16-417) (His tag)

Catalog No. ABIN1096227

This Recombinant CPB1 protein is expressed in Human Cells.

Quick Overview for CPB1 Protein (AA 16-417) (His tag) (ABIN1096227)

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 16-417

Purification tag / Conjugate: This CPB1 protein is labelled with His tag.

Purpose: Recombinant Human Carboxypeptidase B/CPB1 (C-6His)

Sequence: HHGGEHFEGE KVFRVNVEDE NHINIIRELA STTQIDFWKP DSVTQIKPHS TVDFRVKAED TVTVENVLKQ NELQYKVLIS NLRNVVEAQF DSRVRATGHS YEKYNKWETI EAWTQQVATE NPALISRSVI GTTFEGRAIY LLKVGKAGQN KPAIFMDCGF HAREWISPAF CQWFVREAVR TYGREIQVTE LLDKLDFYVL PVLNIDGYIY TWTKSRFWRK TRSTHTGSSC IGTDPNRNFD AGWCEIGASR NPCDETYCGP AAESEKETKA LADFIRNKLS SIKAYLTIHS YSQMMIYPYS YAYKLGENNA ELNALAKATV KELASLHGTK YTYGPGATTI YPAAGGSDDW AYDQGIRYSF TFELRDTGRY GFLLPESQIR ATCEETFLAI KYVASYVLEH LYVDHHHHHH

Characteristics: Recombinant Human Carboxypeptidase B/CPB1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (His16-Tyr417) of Human CPB1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: cpb1

Sub Type: Fusionprotein

Background: Carboxypeptidase B1 is an exopeptidase which specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis and pancreatic graft rejection.Human CPB1 consists of a signal peptide, a pro region, and a mature chain. The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.
Alternative Names: Carboxypeptidase B, Pancreas-Specific Protein, PASP, CPB1, CPB, PCPB

Molecular Weight: 46.94 kDa

UniProt: P15086