CD177 Protein (AA 22-407) (His tag)

Catalog No. ABIN1096299

Product Details

Target: CD177 (CD177 Molecule (CD177))

Protein Type: Recombinant

Protein Characteristics: AA 22-407

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CD177 protein is labelled with His tag.

Purpose: Recombinant Human PRV1/CD177(C-6His)

Sequence: LLCQFGTVQH VWKVSDLPRQ WTPKNTSCDS GLGCQDTLML IESGPQVSLV LSKGCTEAKD QEPRVTEHRM GPGLSLISYT FVCRQEDFCN NLVNSLPLWA PQPPADPGSL RCPVCLSMEG CLEGTTEEIC PKGTTHCYDG LLRLRGGGIF SNLRVQGCMP QPGCNLLNGT QEIGPVGMTE NCNRKDFLTC HRGTTIMTHG NLAQEPTDWT TSNTEMCEVG QVCQETLLLL DVGLTSTLVG TKGCSTVGAQ NSQKTTIHSA PPGVLVASYT HFCSSDLCNS ASSSSVLLNS LPPQAAPVPG DRQCPTCVQP LGTCSSGSPR MTCPRGTTHC YDGYIHLSGG GLSTKMSIQG CVAQPSSFLL NHTRQIGIFS AREKRDVQPP ASQHEGVDHH HHHH

Characteristics: Recombinant Human CD177 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Leu22-Gly407) of Human CD177 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: cd177 (CD177 Products)

Synonyms: HNA-2a Protein, HNA2A Protein, NB1 Protein, NB1 GP Protein, PRV-1 Protein, PRV1 Protein, RGD1562941 Protein, CD177 Protein, MGC157159 Protein, 1190003K14Rik Protein, Pdp3 Protein, CD177 molecule Protein, CD177 antigen Protein, CD177 Protein, Cd177 Protein

Sub Type: Fusionprotein

Background: CD177 is polymorphic and has at least two alleles: PRV1 and NB1. Human PRV1 is a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein that belongs to the uPAR/CD59/Ly6 family of receptors. PRV1 is expressed by neutrophils and neutrophil precursors,and changes in expression serve as diagnostic markers for myeloproliferative disorders such as polycythemia vera and essential thrombocythemia. PRV1 may also be expressed by Erythroblasts, B cells, and Monocytes. NB1, a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia. It is reported that CD177 functions as a novel heterophilic binding partner that engages PECAM-1 in membrane-proximal IgD6.
Alternative Names: CD177 Antigen, Human Neutrophil Alloantigen 2a, HNA-2a, NB1 Glycoprotein, NB1 GP, Polycythemia Rubra Vera Protein 1, PRV-1, CD177, NB1, PRV1

Molecular Weight: 42.33 kDa

UniProt: Q8N6Q3