CD8B Protein (AA 22-170) (His tag)

Catalog No. ABIN1096341

Product Details

Target: CD8B (CD8b Molecule (CD8B))

Protein Type: Recombinant

Protein Characteristics: AA 22-170

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CD8B protein is labelled with His tag.

Purpose: Recombinant Human CD8 β Chain/CD8B (C-6His)

Sequence: LQQTPAYIKV QTNKMVMLSC EAKISLSNMR IYWLRQRQAP SSDSHHEFLA LWDSAKGTIH GEEVEQEKIA VFRDASRFIL NLTSVKPEDS GIYFCMIVGS PELTFGKGTQ LSVVDFLPTT AQPTKKSTLK KRVCRLPRPE TQKGPLCSPV DHHHHHH

Characteristics:

Recombinant Human CD8 β Chain/CD8B (C-6His)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: T-Cell Surface Glycoprotein CD8beta Chain/CD8B (CD8B Products)

Synonyms: Cd8b Protein, Ly-3 Protein, Ly-C Protein, Lyt-3 Protein, CD8B1 Protein, LEU2 Protein, LY3 Protein, LYT3 Protein, P37 Protein, Cd8b1 Protein, CD8b molecule Protein, CD8 antigen, beta chain 1 Protein, Cd8b Protein, CD8B Protein, Cd8b1 Protein

Background: Recombinant Human T-Cell Surface Glycoprotein CD8β Chain/CD8B is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Leu22-Pro170) of Human CD8B fused with a polyhistidine tag at the C-terminus.
T-Cell Surface Glycoprotein CD8β Chain (CD8 Antigen) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 Antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two α chains, or a heterodimer composed of one α and one β chain. Both α and β chains share significant homology to immunoglobulin variable light chains. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

Molecular Weight: 17.8 kDa

UniProt: P10966

Pathways: TCR Signaling