Chitotriosidase 1 Protein (AA 22-466) (His tag)
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- Target See all Chitotriosidase 1 (CHIT1) Proteins
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-466
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This Chitotriosidase 1 protein is labelled with His tag.
- Purpose
- Recombinant Human Chitotriosidase-1/CHIT1 (C-6His)
- Sequence
- AKLVCYFTNW AQYRQGEARF LPKDLDPSLC THLIYAFAGM TNHQLSTTEW NDETLYQEFN GLKKMNPKLK TLLAIGGWNF STQKFTDMVA TANNRQTFVN SAIRFLRKYS FDGLDLDWEY PGSQGSPAVD KERFTTLVQD LANAFQQEAQ TSGKERLLLS AAVPAGQTYV DAGYEVDKIA QNLDFVNLMA YDFHGSWEKV TGHNSPLYKR QEESGAAASL NVDAAVQQWL QKGTPASKLI LGMPTYGRSF TLASSSDTRV GAPATGSGTP GPFTKEGGML AYYEVCSWKG ATKQRIQDQK VPYIFRDNQW VGFDDVESFK TKVSYLKQKG LGGAMVWALD LDDFAGFSCN QGRYPLIQTL RQELSLPYLP SGTPELEVPK PGQPSEPEHG PSPGQDTFCQ GKADGLYPNP RERSSFYSCA GGRLFQQSCP TGLVFSNSCK CCTWNVDHHH HHH
- Characteristics
- Recombinant Human Chitotriosidase-1/CHIT1 produced by transfected human cells is a secreted protein with sequence (Ala22-Asn466) of Human CHIT1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Alternative Name
- chitotriosidase (CHIT1 Products)
- Synonyms
- CHI3 Protein, CHIT Protein, CHITD Protein, CHIT1 Protein, chio Protein, 2300002L19Rik Protein, chitinase 1 Protein, chitinase 1 (chitotriosidase) Protein, CHIT1 Protein, chit1 Protein, Chit1 Protein
- Sub Type
- Fusionprotein
- Background
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Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly, respectively.
Alternative Names: Chitotriosidase-1, Chitinase-1, CHIT1 - Molecular Weight
- 50.43 kDa
- UniProt
- Q13231
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