PPIE Protein (AA 1-301) (His tag)

Catalog No. ABIN1096482

Product Details

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Protein Type: Recombinant

Protein Characteristics: AA 1-301

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPIE protein is labelled with His tag.

Purpose: Recombinant Human Cyclophilin E/PPIase E/PPIE (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD WLKKFSGKTL EENKEEEGSE PPKAETQEGE PIAKKARSNP QVYMDIKIGN KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVIIADCGEY V

Characteristics: Recombinant Human Cyclophilin E is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val301) of Human Cyclophilin E fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Alternative Name: Cyclophilin-E (PPIE Products)

Synonyms: zgc:112471 Protein, CG4886 Protein, CYP33 Protein, Cyp33 Protein, Dcyp33 Protein, Dmel\\CG4886 Protein, PPIE Protein, cyp-33 Protein, cyp33 Protein, cype Protein, CYP-33 Protein, 2010010D16Rik Protein, Ab1-210 Protein, peptidylprolyl isomerase E (cyclophilin E) Protein, cyclophilin-33 Protein, peptidylprolyl isomerase E Protein, peptidylprolyl isomerase E (cyclophilin E) L homeolog Protein, ppie Protein, cyp33 Protein, PPIE Protein, ppie.L Protein, Ppie Protein

Sub Type: Fusionprotein

Background: Peptidyl-prolyl cis-trans isomerase E, also known as Cyclophilin E, Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily. PPIE found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. PPIE contains one PPIase cyclophilin-type domain and one RRM (RNA recognition motif) domain. PPIE accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIE combines RNA-binding and PPIase activities. It may be involved in muscle- and brain-specific processes and pre-mRNA splici
Alternative Names: Peptidyl-Prolyl Cis-Trans Isomerase E, PPIase E, Cyclophilin E, Cyclophilin-33, Rotamase E, PPIE, CYP33

Molecular Weight: 35.6 kDa

UniProt: Q9UNP9