CST5 Protein (AA 21-142) (His tag)

Catalog No. ABIN1096488

Product Details

Target: CST5 (Cystatin D (CST5))

Protein Type: Recombinant

Protein Characteristics: AA 21-142

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This CST5 protein is labelled with His tag.

Purpose: Recombinant Human Cystatin D/CSTD/CST5 (C-6His)

Sequence: GSASAQSRTL AGGIHATDLN DKSVQCALDF AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KVVDHHHHHH

Characteristics: Recombinant Human Cystatin D is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (G21-V142) of Human Cystatin D fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CST5 (Cystatin D (CST5))

Alternative Name: cystatin-d (CST5 Products)

Synonyms: Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein

Sub Type: Fusionprotein

Background: Cystatin-D is a protein that in humans is encoded by the CST5 gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity.
Alternative Names: Cystatin-D, Cystatin-5, CST5

Molecular Weight: 14.9 kDa

UniProt: P28325