DCUN1D1 Protein (AA 1-259) (His tag)

Catalog No. ABIN1096518

Product Details

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Protein Type: Recombinant

Protein Characteristics: AA 1-259

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DCUN1D1 protein is labelled with His tag.

Purpose: Recombinant Human DCN1-Like Protein 1/DCUN1D1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL YIRESVKGSL DRKKLEQLYN RYKDPQDENK IGIDGIQQFC DDLALDPASI SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL LEHHKRSIPK DTWNLLLDFS TMIADDMSNY DEEGAWPVLI DDFVEFARPQ IAGTKSTTV

Characteristics: Recombinant Human DCN1-Like Protein 1/DCUN1D1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val259) of Human DCUN1D1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))

Alternative Name: DCUN1D1 (DCUN1D1 Products)

Synonyms: fd19a01 Protein, zgc:66414 Protein, wu:fd19a01 Protein, dcun1l1 Protein, rp42 Protein, sccro Protein, scro Protein, tes3 Protein, DCNL1 Protein, DCUN1L1 Protein, RP42 Protein, SCCRO Protein, SCRO Protein, Tes3 Protein, Rp42 Protein, pTes3 Protein, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) Protein, DCN1-like protein 1 Protein, DCN1, defective in cullin neddylation 1, domain containing 1 L homeolog Protein, defective in cullin neddylation 1 domain containing 1 Protein, dcun1d1 Protein, dcnl1 Protein, dcun1d1.L Protein, DCUN1D1 Protein, Dcun1d1 Protein

Sub Type: Fusionprotein

Background: DCN1-Like Protein 1 is a protein containing 1 DCUN1 domain and 1 UBA-like domain. DCN1-Like Protein 1 contains part of an E3 Ubiquitin Ligase Complex for Neddylation. It is required for Neddylation of Cullin components of E3 Cullin-RING Ubiquitin Ligase complexes. It enhances the rate of Cullins Neddylation. DCN1-Like Protein 1 recruits the NEDD8-charged E2 Enzyme to the Cullin component. DCUN1D1 is involved in the release of inhibitory effects of CAND1 on the Cullin-RING Ligase E3 complex assembly and activity. It acts also as an oncogene facilitating malignant transformation and carcinogenic progression.
Alternative Names: DCN1-Like Protein 1, DCUN1 Domain-Containing Protein 1, Defective in Cullin Neddylation Protein 1-Like Protein 1, Squamous Cell Carcinoma-Related Oncogene, DCUN1D1, DCUN1L1, RP42, SCCRO

Molecular Weight: 32.3 kDa

UniProt: Q96GG9