EDIL3 Protein (AA 17-480) (His tag)

Catalog No. ABIN1096549

Product Details

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Protein Type: Recombinant

Protein Characteristics: AA 17-480

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This EDIL3 protein is labelled with His tag.

Purpose: Recombinant Human EDIL3/Del-1 (C-6His)

Sequence: VPQFGKGDIC DPNPCENGGI CLPGLADGSF SCECPDGFTD PNCSSVVEVA SDEEEPTSAG PCTPNPCHNG GTCEISEAYR GDTFIGYVCK CPRGFNGIHC QHNINECEVE PCKNGGICTD LVANYSCECP GEFMGRNCQY KCSGPLGIEG GIISNQQITA SSTHRALFGL QKWYPYYARL NKKGLINAWT AAENDRWPWI QINLQRKMRV TGVITQGAKR IGSPEYIKSY KIAYSNDGKT WAMYKVKGTN EDMVFRGNID NNTPYANSFT PPIKAQYVRL YPQVCRRHCT LRMELLGCEL SGCSEPLGMK SGHIQDYQIT ASSIFRTLNM DMFTWEPRKA RLDKQGKVNA WTSGHNDQSQ WLQVDLLVPT KVTGIITQGA KDFGHVQFVG SYKLAYSNDG EHWTVYQDEK QRKDKVFQGN FDNDTHRKNV IDPPIYARHI RILPWSWYGR ITLRSELLGC TEEEVDHHHH HH

Characteristics: Recombinant Human EGF-Like Repeat and Discoidin I-Like Domain-Containing Protein 3/EDIL3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Val17-Glu480) of Human EDIL3 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Alternative Name: edil3 (DEL1 Products)

Synonyms: si:dkey-84i7.1 Protein, Del1 Protein, XDel1 Protein, DEL1 Protein, Del-1 Protein, EGF-like repeats and discoidin I-like domains 3a Protein, EGF like repeats and discoidin domains 3 L homeolog Protein, EGF like repeats and discoidin domains 3 Protein, EGF-like repeats and discoidin I-like domains 3 Protein, edil3a Protein, edil3.L Protein, EDIL3 Protein, Edil3 Protein

Sub Type: Fusionprotein

Background: EGF-Like Repeat and Discoidin I-Like Domain-Containing Protein 3 (EDIL3) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development. EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. EDIL3 has been found to be re-expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis. EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through upregulation of Bcl-2 expression. EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion. Human EDIL3 is synthesized as a precursor with a 16 amino acid signal sequence and a 464 amino acid mature chain.
Alternative Names: EGF-Like Repeats and Discoidin I-Like Domains 3, EDIL3

Molecular Weight: 53.09 kDa

UniProt: Q8N610