Grancalcin Protein (AA 1-218) (GST tag)
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- Target See all Grancalcin (GCA) Proteins
- Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-218
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Grancalcin protein is labelled with GST tag.
- Purpose
- Recombinant Human Grancalcin/GCA (N-GST)
- Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAYP GYGGGFGNFS IQVPGMQMGQ PVPETGPAIL LDGYSGPAYS DTYSSAGDSV YTYFSAVAGQ DGEVDAEELQ RCLTQSGING TYSPFSLETC RIMIAMLDRD HTGKMGFNAF KELWAALNAW KENFMTVDQD GSGTVEHHEL RQAIGLMGYR LSPQTLTTIV KRYSKNGRIF FDDYVACCVK LRALTDFFRK RDHLQQGSAN FIYDDFLQGT MAI
- Characteristics
- Recombinant Human Grancalcin/GCA is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys218) of Human Grancalcin fused with a GST tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GCA Protein
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Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (His tag)
GCA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-217) protein (GST tag)GCA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Grancalcin, EF-Hand Calcium Binding Protein (GCA) (AA 1-220) protein (Strep Tag)Crystallography grade GCA Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Grancalcin, EF-Hand Calcium Binding Protein (GCA) protein (Myc-DYKDDDDK Tag)GCA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 500 mM Nacl, pH 8.5 .
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))
- Alternative Name
- grancalcin (GCA Products)
- Synonyms
- Grancalcin Protein, 5133401E04Rik Protein, AI573844 Protein, GCL Protein, zgc:92027 Protein, grancalcin Protein, Grancalcin Protein, zgc:92027 Protein, GCA Protein, gran Protein, LOC100280890 Protein, Gca Protein
- Sub Type
- Fusionprotein
- Background
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Grancalcin (GCA) is a member of the penta EF hand subfamily which includes sorcin, calpain and ALG2. Grancalcin is highly expressed bone marrow and also can detected in neutrophils and macrophages. Grancalcin interacts with L-plastin which known to have actin bundling activity. It indicates that Grancalcin may play an important role in the adhesion of neutrophils to fibronectin. Furthermore, Grancalcin localization is dependent upon calcium and magnesium. It associates with both the granule and membrane fractions, which suggested a role for grancalcin in granule-membrane fusion and degranulation.
Alternative Names: Grancalcin, GCA, GCL - Molecular Weight
- 50.3 kDa
- UniProt
- P28676
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