UBE2A Protein (AA 1-152) (GST tag,His tag)

Catalog No. ABIN1096837

Product Details

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Protein Type: Recombinant

Protein Characteristics: AA 1-152

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2A protein is labelled with GST tag,His tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 A/UBE2A (N-GST, 6His)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 A/UBE2A is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Cys152) of Human UBE2A fused with a GST tag and a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2A (ube2a) (Ubiquitin-Conjugating Enzyme E2A (ube2a))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 A/UBE2A (ube2a Products)

Synonyms: BHR6A Protein, HR6A Protein, hhr6a Protein, rad6a Protein, ubc2 Protein, mp:zf637-2-000771 Protein, si:bz46j2.4 Protein, wu:fa01h11 Protein, HHR6A Protein, MRXS30 Protein, MRXSN Protein, RAD6A Protein, UBC2 Protein, Mhr6a Protein, ubiquitin conjugating enzyme E2 A Protein, ubiquitin conjugating enzyme E2 A L homeolog Protein, ubiquitin-conjugating enzyme E2A (RAD6 homolog) Protein, ubiquitin-conjugating enzyme E2A Protein, UBE2A Protein, ube2a.L Protein, ube2a Protein, Ube2a Protein

Sub Type: Fusionprotein

Background: Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 A, RAD6 Homolog A, HR6A, hHR6A, Ubiquitin Carrier Protein A, Ubiquitin-Protein Ligase A, UBE2A, RAD6A

Molecular Weight: 49 kDa

UniProt: P49459