MIA Protein (AA 25-131) (His tag)

Catalog No. ABIN1097143

Product Details

Target: MIA (Melanoma Inhibitory Activity (MIA))

Protein Type: Recombinant

Protein Characteristics: AA 25-131

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MIA protein is labelled with His tag.

Purpose: Recombinant Human Melanoma Inhibitory Activity Protein/MIA (C-6His)

Sequence: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQLE HHHHHH

Characteristics: Recombinant Human Melanoma Inhibitory Activity Protein/MIA is produced with our E. coli expression system. The target protein is expressed with sequence (Gly25-Gln131) of Human MIA.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: melanoma-inhibitory-activity-protein (MIA Products)

Synonyms: CD-RAP Protein, Mia1 Protein, Cdrap Protein, zgc:194157 Protein, mia Protein, CDRAP Protein, Fdp Protein, MIA Protein, MIAL Protein, melanoma inhibitory activity Protein, otoraplin Protein, cilia and flagella associated protein 100 Protein, MIA Protein, Mia Protein, mia Protein, Otor Protein, CFAP100 Protein

Background: Melanoma Inhibitory Activity Protein (MIA) is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells, which was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family: MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion and promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. The protein MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Alternative Names: Melanoma-Derived Growth Regulatory Protein, Melanoma Inhibitory Activity Protein, MIA

Molecular Weight: 13.31 kDa

UniProt: Q16674