NADK Protein (AA 64-446) (His tag)
Quick Overview for NADK Protein (AA 64-446) (His tag) (ABIN1097191)
Target
See all NADK ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 64-446
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Purification tag / Conjugate
- This NADK protein is labelled with His tag.
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Purpose
- Recombinant Human NAD Kinase/NADK (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MSLHGPCPVT TFGPKACVLQ NPQTIMHIQD PASQRLTWNK SPKSVLVIKK MRDASLLQPF KELCTHLMEE NMIVYVEKKV LEDPAIASDE SFGAVKKKFC TFREDYDDIS NQIDFIICLG GDGTLLYASS LFQGSVPPVM AFHLGSLGFL TPFSFENFQS QVTQVIEGNA AVVLRSRLKV RVVKELRGKK TAVHNGLGEK GSQAAGLDMD VGKQAMQYQV LNEVVIDRGP SSYLSNVDVY LDGHLITTVQ GDGVIVSTPT GSTAYAAAAG ASMIHPNVPA IMITPICPHS LSFRPIVVPA GVELKIMLSP EARNTAWVSF DGRKRQEIRH GDSISITTSC YPLPSICVRD PVSDWFESLA QCLHWNVRKK QAHFEEEEEE EEEG
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Characteristics
- Recombinant Human NAD Kinase/NADK is produced by our E. coli expression system. The target protein is expressed with sequence (Ser64-Gly446) of Human NADK fused with a 6His tag at the N-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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NAD Kinase (NADK) (AA 1-439) protein (His tag)
custom-made NADK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 1-446) protein (His tag)custom-made NADK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 64-298) protein (His tag)NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 1 mM DTT, pH 7.5.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- NADK (NAD Kinase (NADK))
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Alternative Name
- NAD Kinase/NADK
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Sub Type
- Fusionprotein
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Background
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NAD Kinase (NADK) is an enzyme that belongs to the NAD Kinase family. It is a widely expressed enzyme, but it is not detected in skeletal muscle. NADK converts Nicotinamide Adenine Dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.
Alternative Names: NAD Kinase, Poly(P)/ATP NAD Kinase, NADK -
Molecular Weight
- 44.4 kDa
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UniProt
- O95544
Target
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