NME1 Protein (AA 1-152) (His tag)
-
- Target See all NME1 Proteins
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-152
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NME1 protein is labelled with His tag.
- Purpose
- Recombinant Human Nucleoside Diphosphate Kinase A/NDPKA (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
- Characteristics
- Recombinant Human Nucleoside Diphosphate Kinase A/NDPKA is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Glu152) of Human NDPKA fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NME1 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
-
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
-
- Target
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
- Alternative Name
- Nucleoside Diphosphate Kinase A/NDPKA (NME1 Products)
- Synonyms
- AWD Protein, GAAD Protein, NB Protein, NBS Protein, NDKA Protein, NDPK-A Protein, NDPKA Protein, NM23 Protein, NM23-H1 Protein, AL024257 Protein, NM23-M1 Protein, NM23A Protein, NDPK-Z1 Protein, NM23-B Protein, NM23-Z1 Protein, ndpkz1 Protein, nm23b Protein, nme1 Protein, nme2 Protein, nme2b1 Protein, NME1 Protein, NME1-NME2 Protein, NM23-C1 Protein, ndpk-b Protein, ndpkb Protein, nm23-h2 Protein, nm23ndk Protein, puf Protein, NBR-A Protein, NBR-B Protein, NME1-1 Protein, nm23ndk-a Protein, NDK I Protein, NDPK I Protein, T30A10.80 Protein, T30A10_80 Protein, NDP kinase I Protein, NME/NM23 nucleoside diphosphate kinase 1 Protein, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 Protein, non-metastatic cells 1, protein (NM23A) expressed in Protein, NME/NM23 nucleoside diphosphate kinase 2 S homeolog Protein, NME/NM23 nucleoside diphosphate kinase 2 L homeolog Protein, nucleoside diphosphate kinase Protein, nucleoside diphosphate kinase 1 Protein, NME1 Protein, Nme1 Protein, nme2b.1 Protein, nme2.S Protein, nme2.L Protein, LOC547870 Protein, NDPK1 Protein, LOC107790563 Protein
- Sub Type
- Fusionprotein
- Background
-
Nucleoside-Diphosphate Kinases (NDKs) are enzymes that catalyze the exchange of phosphate groups between different nucleoside diphosphates. NDKs Possesse nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3-5 exonuclease activities. NDKs involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression and required for neural development including neural patterning and cell fate determination. Prokaryotic NDK forms a functional homotetramer.There are two isoforms of NDK in humans: NDK-A and NDK-B. Both have very similar structure, and can combine in any proportion to form functional NDK hexamers.
Alternative Names: Nucleoside Diphosphate Kinase A, NDK A, NDP Kinase A, Granzyme A-Activated DNase, GAAD, Metastasis Inhibition Factor nm23, Tumor Metastatic Process-Associated Protein, nm23-H1, NME1, NDPKA, NM23 - Molecular Weight
- 19.3 kDa
- UniProt
- P15531
- Pathways
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
-