OTUB2 Protein (AA 1-234) (GST tag)

Catalog No. ABIN1097272

Product Details

Target: OTUB2 (OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2))

Protein Type: Recombinant

Protein Characteristics: AA 1-234

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This OTUB2 protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSE TSFNLISEKC DILSILRDHP ENRIYRRKIE ELSKRFTAIR KTKGDGNCFY RALGYSYLES LLGKSREIFK FKERVLQTPN DLLAAGFEEH KFRNFFNAFY SVVELVEKDG SVSSLLKVFN DQSASDHIVQ FLRLLTSAFI RNRADFFRHF IDEEMDIKDF CTHEVEPMAT ECDHIQITAL SQALSIALQV EYVDEMDTAL NHHVFPEAAT PSVYLLYKTS HYNILYAADK H

Characteristics:

Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 (N-GST)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: OTUB2 (OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2))

Alternative Name: Ubiquitin Thioesterase OTUB2/OTUB2 (OTUB2 Products)

Synonyms: OTUB2 Protein, otb2 Protein, otu2 Protein, C14orf137 Protein, OTB2 Protein, OTU2 Protein, 2010015L18Rik Protein, 4930586I02Rik Protein, AI413508 Protein, AW557219 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 2 Protein, OTU deubiquitinase, ubiquitin aldehyde binding 2 S homeolog Protein, OTU domain, ubiquitin aldehyde binding 2 Protein, OTUB2 Protein, otub2.S Protein, otub2 Protein, Otub2 Protein

Background: Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-His234) of Human OTUB2 fused with a GST tag at the N-terminus.
Ubiquitin Thioesterase OTUB2 belongs to the peptidase C65 family, functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro, may plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB2 is expressed at higher levels in the brain.

Molecular Weight: 53.6 kDa

UniProt: Q96DC9