PMVK Protein (AA 1-192) (His tag)
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- Target See all PMVK Proteins
- PMVK (Phosphomevalonate Kinase (PMVK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-192
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PMVK protein is labelled with His tag.
- Purpose
- Recombinant Human Phosphomevalonate Kinase/PMVK (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL
- Characteristics
- Recombinant Human Phosphomevalonate Kinase/PMVK is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Leu192) of Human PMVK fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PMVK Protein
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Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (His tag)
PMVK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (GST tag)PMVK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphomevalonate Kinase (PMVK) (AA 1-192) protein (Strep Tag)Crystallography grade PMVK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Phosphomevalonate Kinase (PMVK) protein (Myc-DYKDDDDK Tag)PMVK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PMVK (Phosphomevalonate Kinase (PMVK))
- Alternative Name
- Phosphomevalonate Kinase/PMVK (PMVK Products)
- Synonyms
- MPK_Bm Protein, F5M6.9 Protein, F5M6_9 Protein, zgc:162701 Protein, HUMPMKI Protein, PMK Protein, PMKA Protein, PMKASE Protein, 1110011E12Rik Protein, 2900002L22Rik Protein, AV001327 Protein, phosphomevalonate kinase Protein, GHMP kinase family protein Protein, PMVK Protein, Mpk Protein, AT1G31910 Protein, CpipJ_CPIJ015413 Protein, Bm1_05275 Protein, pmvk Protein, LOAG_11811 Protein, Tsp_06629 Protein, LOC100282968 Protein, Pmvk Protein
- Sub Type
- Fusionprotein
- Background
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Phosphomevalonate kinase (PMVK) is a cytosolic enzyme. PMVK can be highly expressed in the heart,skeletal muscle, liver, pancreas, and kidney, it is expressed at lower levels in the brain, lung, and placenta. Induced by sterol, PMVK takes part in isopentenyl diphosphate biosynthesis through the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
Alternative Names: Phosphomevalonate Kinase, PMKase, hPMK, PMVK, PMKI - Molecular Weight
- 24.2 kDa
- UniProt
- Q15126
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