PMM2 Protein (AA 1-246) (His tag)

Catalog No. ABIN1097361

This Recombinant PMM2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for PMM2 Protein (AA 1-246) (His tag) (ABIN1097361)

Target: PMM2 (Phosphomannomutase 2 (PMM2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-246

Purification tag / Conjugate: This PMM2 protein is labelled with His tag.

Purpose: Recombinant Human Phosphomannomutase 2/PMM2 (C-6His)

Sequence: MAAPGPALCL FDVDGTLTAP RQKITKEMDD FLQKLRQKIK IGVVGGSDFE KVQEQLGNDV VEKYDYVFPE NGLVAYKDGK LLCRQNIQSH LGEALIQDLI NYCLSYIAKI KLPKKRGTFI EFRNGMLNVS PIGRSCSQEE RIEFYELDKK ENIRQKFVAD LRKEFAGKGL TFSIGGQISF DVFPDGWDKR YCLRHVENDG YKTIYFFGDK TMPGGNDHEI FTDPRTMGYS VTAPEDTRRI CELLFSLEHH HHHH

Characteristics: Recombinant Human Phosphomannomutase 2/PMM2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser246) of Human PMM2 fused with a His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PMM2 (Phosphomannomutase 2 (PMM2))

Alternative Name: PMM2

Sub Type: Fusionprotein

Background: Phosphomannomutase 2 (PMM2) is an enzyme that is a member of the highly variable methyltransferase superfamily. PMM2 is a cytoplasmic protein and catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate.In addition, PMM2 involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose that required for a number of critical mannosyl transfer reactions. Defects in PMM2 can results in congenital disorder of glycosylation type 1A (CDG1A). Congenital disorders of glycosylation are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation.
Alternative Names: Phosphomannomutase 2, PMM 2, PMM2

Molecular Weight: 29.1 kDa

UniProt: O15305