PPM1G Protein (AA 1-323, Catalytic Subunit) (His tag)

Catalog No. ABIN1097367

Product Details

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Protein Type: Recombinant

Protein Characteristics: Catalytic Subunit, AA 1-323

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPM1G protein is labelled with His tag.

Purpose: Recombinant Human Protein Phosphatase 1C Catalytic Subunit/PPP1CC (N, C-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKKLEHHHHH H

Characteristics: Recombinant Human Serine/Threonine-Protein Phosphatase PP1-gamma Catalytic Subunit/PPP1CC is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Lys323) of Human PPP1CC fused with a 6His tag at both the N-terminus and C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, pH 8.0 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Alternative Name: Protein Phosphatase 1C (PPM1G Products)

Synonyms: AI385652 Protein, Fin13 Protein, PP2CG Protein, PP2CGAMMA Protein, PPP2CG Protein, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent 1G Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1G Protein, Ppm1g Protein, PPM1G Protein

Sub Type: Fusionprotein

Background: Serine/Threonine-Protein Phosphatase PP1-Ypsilon Catalytic Subunit (PPP1CC) is a member of the PPP phosphatase family. It is essential for cell division, participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CC colocalizes with SPZ1 in the nucleus, with URI1 at mitochondrion, Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. As a cofactor, PPP1CC binds one iron ion and one manganese ion per subunit.. In addition, PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
Alternative Names: Serine/Threonine-Protein Phosphatase PP1-Gamma Catalytic Subunit, PP-1G, Protein Phosphatase 1C Catalytic Subunit, PPP1CC

Molecular Weight: 40.2 kDa

UniProt: P36873