PPCDC Protein (AA 1-204) (His tag)
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- Target See all PPCDC Proteins
- PPCDC (phosphopantothenoylcysteine Decarboxylase (PPCDC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-204
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPCDC protein is labelled with His tag.
- Purpose
- Recombinant Human Phosphopantothenoylcysteine Decarboxylase/PPC-DC (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS
- Characteristics
- Recombinant Human Phosphopantothenoylcysteine Decarboxylase/PPC-DC is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser204) of Human PPCDC fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PPCDC Protein
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phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (GST tag)
PPCDC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (Strep Tag)Crystallography grade PPCDC Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (Strep Tag)Crystallography grade PPCDC Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
phosphopantothenoylcysteine Decarboxylase (PPCDC) protein (Myc-DYKDDDDK Tag)PPCDC Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PPCDC (phosphopantothenoylcysteine Decarboxylase (PPCDC))
- Alternative Name
- PPC-DC (PPCDC Products)
- Synonyms
- MDS018 Protein, 1810057I13Rik Protein, 8430432M10Rik Protein, RGD1306267 Protein, phosphopantothenoylcysteine decarboxylase Protein, PPCDC Protein, Ppcdc Protein
- Sub Type
- Fusionprotein
- Background
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Phosphopantothenoylcysteine Decarboxylase (PPC-DC) is an essential enzyme in the biosynthesis of Coenzyme A and catalyzes the decarboxylation of PPC to Phosphopantetheine. PPC-DC catalyzes the decarboxylation of the Cysteine moiety of 4-Phosphopantothenoylcysteine (PPC) to form 4-Phosphopantetheine (PPantSH), this reaction forms part of the biosynthesis of Coenzyme A. The enzyme is a member of the larger family of Cysteine Decarboxylases including the Lantibiotic-Biosynthesizing enzymes EpiD and MrsD, all of which use a tightly bound Flavin cofactor to oxidize the Thiol moiety of the substrate to a Thioaldehyde.
Alternative Names: Phosphopantothenoylcysteine Decarboxylase, PPC-DC, PPCDC, COAC - Molecular Weight
- 24.6 kDa
- UniProt
- Q96CD2
- Pathways
- Ribonucleoside Biosynthetic Process
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