SUMF1 Protein (AA 34-374) (His tag)
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- Target See all SUMF1 Proteins
- SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 34-374
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This SUMF1 protein is labelled with His tag.
- Purpose
- Recombinant Human Sulfatase Modifying Factor 1/SUMF1 (C-6His)
- Sequence
- SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM DVDHHHHHH
- Characteristics
- Recombinant Human Sulfatase Modifying Factor 1/SUMF1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser34-Asp374) of Human SUMF1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SUMF1 Protein
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Sulfatase Modifying Factor 1 (SUMF1) (AA 101-216) protein (His-IF2DI Tag)
SUMF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sulfatase Modifying Factor 1 (SUMF1) (AA 91-374) protein (His tag)SUMF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Sulfatase Modifying Factor 1 (SUMF1) (AA 89-188) protein (GST tag)SUMF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sulfatase Modifying Factor 1 (SUMF1) (AA 34-374) protein (His tag)Crystallography grade SUMF1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sulfatase Modifying Factor 1 (SUMF1) (AA 32-372) protein (His tag)Crystallography grade SUMF1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Sulfatase Modifying Factor 1 (SUMF1) proteinSUMF1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Sulfatase Modifying Factor 1 (SUMF1) protein (Myc-DYKDDDDK Tag)SUMF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 2 mM CaCl2, 10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))
- Alternative Name
- sumf1 (SUMF1 Products)
- Synonyms
- AAPA3037 Protein, FGE Protein, AA543204 Protein, AI463102 Protein, AI851573 Protein, sulfatase modifying factor 1 Protein, SUMF1 Protein, Sumf1 Protein
- Sub Type
- Fusionprotein
- Background
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Human Sulfatase Modifying Factor 1 (SUMF1) is a 42kDa protein. SUMF1 is a Ca2+-binging member of the sulfatase-modifying factor family. SUMF1 is a soluble ER lumenal glycoprotein, it converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. Three splice isoforms are known.
Alternative Names: Sulfatase-Modifying Factor 1, C-Alpha-Formylglycine-Generating Enzyme 1, SUMF1, FGE - Molecular Weight
- 38.27 kDa
- UniProt
- Q8NBK3
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