GTF2B Protein (AA 1-316) (GST tag)

Catalog No. ABIN1097625

Product Details

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Protein Type: Recombinant

Protein Characteristics: AA 1-316

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GTF2B protein is labelled with GST tag.

Purpose: Recombinant Human Transcription Initiation Factor IIB/TFIIB/GTF2B (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF PTDFKFDTPV DKLPQL

Characteristics: Recombinant Human Transcription Initiation Factor IIB/TFIIB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu316) of Human TFIIB fused with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: TF2B (GTF2B Products)

Synonyms: CG5193 Protein, Dmel\\CG5193 Protein, TFIIB Protein, Tfiib Protein, TfllB Protein, dTFIIB Protein, dTfIIB Protein, dmTFIIB Protein, gtf2b Protein, tf2b Protein, tfiib Protein, XLTFIIB Protein, TF2B Protein, wu:fc44c11 Protein, zgc:66258 Protein, Transcription factor IIB Protein, transcription initiation factor iib Protein, transcription initiation factor IIB Protein, transcription initiation factor IIB 2 Protein, Transcription initiation factor IIB Protein, general transcription factor 2B S homeolog Protein, general transcription factor 2B L homeolog Protein, general transcription factor IIB Protein, TfIIB Protein, CNF01950 Protein, tfb Protein, CC1G_14689 Protein, HacjB3_18813 Protein, HALXA_RS07110 Protein, HALXA_RS16310 Protein, tf2b Protein, LOC100283348 Protein, gtf2b.S Protein, gtf2b.L Protein, GTF2B Protein, gtf2b Protein, Gtf2b Protein

Sub Type: Fusionprotein

Background: Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
Alternative Names: Transcription Initiation Factor IIB, General Transcription Factor TFIIB, S300-II, GTF2B, TF2B, TFIIB

Molecular Weight: 61.64 kDa

UniProt: Q00403