TNFRSF10B Protein (AA 56-182) (His tag)

Catalog No. ABIN1097685

Product Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Protein Characteristics: AA 56-182

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This TNFRSF10B protein is labelled with His tag.

Purpose: Recombinant Human TRAIL R2/TNFRSF10B/DR5/CD262 (C-6His)

Sequence: ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKEVDH HHHHH

Characteristics: Recombinant Human TNF-Related Apoptosis-Inducing Ligand Receptor 2/TRAIL-R2 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ile56-Glu182) of Human TRAILR2 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: trail-r2 (TNFRSF10B Products)

Synonyms: TNFRSF10B Protein, dr5 Protein, cd262 Protein, killer Protein, trick2 Protein, trickb Protein, xDR-M1 Protein, xDR-M2 Protein, ztnfr9 Protein, trailr2 Protein, trick2a Protein, trick2b Protein, CD262 Protein, DR5 Protein, KILLER Protein, KILLER/DR5 Protein, TRAIL-R2 Protein, TRAILR2 Protein, TRICK2 Protein, TRICK2A Protein, TRICK2B Protein, TRICKB Protein, ZTNFR9 Protein, Ly98 Protein, MK Protein, tumor necrosis factor receptor superfamily, member 10b Protein, tumor necrosis factor receptor superfamily member 10b S homeolog Protein, tumor necrosis factor receptor superfamily member 10b L homeolog Protein, tumor necrosis factor receptor superfamily member 10b Protein, TNF receptor superfamily member 10b Protein, Tnfrsf10b Protein, TNFRSF10B Protein, tnfrsf10b.S Protein, tnfrsf10b.L Protein, tnfrsf10b Protein

Sub Type: Fusionprotein

Background: TNFRSF10B is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. The adapter molecule FADD recruits caspase-8 to the activated receptor and is required for the apoptosis mediated by TNFRSF10B. TNFRSF10B is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. This single-pass transmembrane protein contains two cysteine-rich repeat units in its extracellular region, followed by a transmembrane segment and a cytoplasmic tail containing a typical “death domain”. TNFRSF10B expression is regulated by the tumor suppressor p53. It is also indicated that the activation of NF-kappa-B can be promoted by TNFRSF10B.
Alternative Names: Tumor Necrosis Factor Receptor Superfamily Member 10B, Death Receptor 5, TNF-Related Apoptosis-Inducing Ligand Receptor 2, TRAIL Receptor 2, TRAIL-R2, CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9

Molecular Weight: 15.19 kDa

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity