PRSS22 Protein (AA 33-317) (His tag)
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- Target See all PRSS22 Proteins
- PRSS22 (Protease, serine, 22 (PRSS22))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-317
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PRSS22 protein is labelled with His tag.
- Purpose
- Recombinant Human Tryptase epsilon/Brain-Specific Serine Protease 4/BSSP-4 (C-6His)
- Sequence
- ARIPVPPACG KPQQLNRVVG GEDSTDSEWP WIVSIQKNGT HHCAGSLLTS RWVITAAHCF KDNLNKPYLF SVLLGAWQLG NPGSRSQKVG VAWVEPHPVY SWKEGACADI ALVRLERSIQ FSERVLPICL PDASIHLPPN THCWISGWGS IQDGVPLPHP QTLQKLKVPI IDSEVCSHLY WRGAGQGPIT EDMLCAGYLE GERDACLGDS GGPLMCQVDG AWLLAGIISW GEGCAERNRP GVYISLSAHR SWVEKIVQGV QLRGRAQGGG ALRAPSQGSG AAARSHHHHH H
- Characteristics
- Recombinant Human Brain-Specific Serine Protease 4/BSSP-4 produced by transfected human cells is a secreted protein with sequence(Ala33-Ser317) of Human BSSP-4 Tryptase fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PRSS22 Protein
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Protease, serine, 22 (PRSS22) (AA 33-317) protein (His tag)
Crystallography grade PRSS22 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Protease, serine, 22 (PRSS22) (AA 33-306) protein (His tag)Crystallography grade PRSS22 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Protease, serine, 22 (PRSS22) (AA 33-307) protein (His tag)PRSS22 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE. SDS
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, 10 % Glycerol, pH 4.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PRSS22 (Protease, serine, 22 (PRSS22))
- Alternative Name
- tryptase-epsilon (PRSS22 Products)
- Synonyms
- BSSP-4 Protein, hBSSP-4 Protein, 4733401N09Rik Protein, SP001LA Protein, protease, serine, 22 Protein, protease, serine 22 Protein, Prss22 Protein, PRSS22 Protein
- Sub Type
- Fusionprotein
- Background
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Brain-Specific Serine Protease 4 (BSSP-4) is a serine protease that preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA. BSSP-4 is expressed abundantly in the epithelial cells of the airways, including trachea, esophagus and fetal lung, but scarce in adult lung and expressed at low levels in placenta, pancreas, prostate and thyroid gland. BSSP-4 belongs to the peptidase S1 family and related to trypsin, referentially hydrolyzing substrates after arginine and lysine residues. However, BSSP-4 is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha1-antitrypsin and secretory leukocyte protease inhibitor. BSSP-4 efficiently converts pro-urokinase- type plasminogen activator to its mature, active form.
Alternative Names: Brain-Specific Serine Protease 4, BSSP-4, Serine Protease 22, Serine Protease 26, Tryptase Epsilon, PRSS22, BSSP4, PRSS26 - Molecular Weight
- 31.56 kDa
- UniProt
- Q9GZN4
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