PLAU Protein (AA 21-431) (His tag)
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- Target See all PLAU Proteins
- PLAU (Plasminogen Activator, Urokinase (PLAU))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-431
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PLAU protein is labelled with His tag.
- Purpose
- Recombinant Human Urokinase-Type Plasminogen Activator/uPA/PLAU (C-6His)
- Sequence
- SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLVQECMVHD CADGKKPSSP PEELKFQCGQ KTLRPRFKII GGEFTTIENQ PWFAAIYRRH RGGSVTYVCG GSLISPCWVI SATHCFIDYP KKEDYIVYLG RSRLNSNTQG EMKFEVENLI LHKDYSADTL AHHNDIALLK IRSKEGRCAQ PSRTIQTICL PSMYNDPQFG TSCEITGFGK ENSTDYLYPE QLKMTVVKLI SHRECQQPHY YGSEVTTKML CAADPQWKTD SCQGDSGGPL VCSLQGRMTL TGIVSWGRGC ALKDKPGVYT RVSHFLPWIR SHTKEENGLA LVDHHHHHH
- Characteristics
- Recombinant Human Urokinase-Type Plasminogen Activator is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser21-Leu431) of Human PLAU fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PLAU Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, 2 mM CaCl, 10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PLAU (Plasminogen Activator, Urokinase (PLAU))
- Alternative Name
- Urokinase (PLAU Products)
- Synonyms
- PLAU Protein, u-PA Protein, ATF Protein, BDPLT5 Protein, QPD Protein, UPA Protein, URK Protein, uPA Protein, UPAM Protein, plasminogen activator, urokinase Protein, urokinase-type plasminogen activator Protein, PLAU Protein, CpipJ_CPIJ002131 Protein, CpipJ_CPIJ006543 Protein, CpipJ_CPIJ013396 Protein, CpipJ_CPIJ013623 Protein, Plau Protein
- Sub Type
- Fusionprotein
- Background
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Recombinant Human Urokinase-Type Plasminogen Activator is a serine protease, which specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. Urokinase-Type Plasminogen Activator is a potent marker of invasion and metastasis in many human cancers associated with breast, colon, stomach, bladder, brain, ovary and endometrium. Human Urokinase-Type Plasminogen Activator is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide residues. The single chain molecule is processed into a disulfide-linked two-chain molecule. There exists two forms A chain, the long A chain contains an EGF-like domain that is responsible for binding of the uPA receptor. The B chain corresponds to the catalytic domain.
Alternative Names: Urokinase-Type Plasminogen Activator, U-Plasminogen Activator, uPA, PLAU - Molecular Weight
- 47.41 kDa
- UniProt
- P00749
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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