UBE2G2 Protein (AA 1-165) (GST tag)

Catalog No. ABIN1097730

Product Details

Target: UBE2G2 (Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2))

Protein Type: Recombinant

Protein Characteristics: AA 1-165

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2G2 protein is labelled with GST tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 G2/UBE2G2/UBC7 (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMAG TALKRLMAEY KQLTLNPPEG IVAGPMNEEN FFEWEALIMG PEDTCFEFGV FPAILSFPLD YPLSPPKMRF TCEMFHPNIY PDGRVCISIL HAPGDDPMGY ESSAERWSPV QSVEKILLSV VSMLAEPNDE SGANVDASKM WRDDREQFYK IAKQIVQKSL GL

Characteristics:

Recombinant Human Ubiquitin-Conjugating Enzyme E2 G2/UBE2G2/UBC7 (N-GST)

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2G2 (Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 G2/UBE2G2 (UBE2G2 Products)

Synonyms: UBC7 Protein, 1110003O05Rik Protein, D10Xrf369 Protein, Ubc7p Protein, wu:fu51c05 Protein, wu:fu56g11 Protein, zgc:112077 Protein, ubc7 Protein, ube2g1 Protein, ube2g2 Protein, wu:fi33c04 Protein, zgc:55974 Protein, ubiquitin conjugating enzyme E2 G2 Protein, ubiquitin-conjugating enzyme E2G 2 Protein, ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2 G2 Protein, Ubiquitin-conjugating enzyme E2 G2 Protein, ubiquitin conjugating enzyme E2 G2 L homeolog Protein, ubiquitin-conjugating enzyme E2G 1a (UBC7 homolog, yeast) Protein, UBE2G2 Protein, Ube2g2 Protein, ube2g2 Protein, EDI_023910 Protein, CpipJ_CPIJ018658 Protein, Bm1_47630 Protein, ube2g2.L Protein, ube2g1a Protein

Background: Recombinant Human Ubiquitin-Conjugating Enzyme E2 G2/UBE2G2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu165) of Human UBE2G2 fused with a GST tag at the N-terminus.
Ubiquitin-Conjugating Enzyme E2 G2 (UBE2G2) is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation, which belong to the ubiquitin-conjugating enzyme family. It shares 60 % and 100 % sequence identity with S.cerevisiae Ubc7 and mouse respectively. The UBE2G2 enzyme and the GP78 E3 ligase are active components of endoplasmic reticulum-associated degradation pathway which is essential for the degradation of misfolded ER proteins. The mechanism of K48-linked poly-ubiquitination by UBE2G2/GP78 appears to involve the transfer of preassembled Ub chains from UBE2G2 to lysine residues in a substrate. The E2 and E3 enzymes form a large hetero-oligomer which brings multiple UBE2G2 molecules into close proximity which allows for Ub transfer between neighboring E2s.

Molecular Weight: 45 kDa

UniProt: P60604