Ube2t Protein (AA 1-197) (His tag)
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- Target See all Ube2t Proteins
- Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-197
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Ube2t protein is labelled with His tag.
- Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 T/UBE2T (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV
- Characteristics
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 T/UBE2T is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val197) of Human UBE2T.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product Ube2t Protein
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Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (His tag)
Ube2t Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (GST tag)Ube2t Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (Strep Tag)Crystallography grade Ube2t Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2T (Ube2t) protein (Myc-DYKDDDDK Tag)Ube2t Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))
- Alternative Name
- Ubiquitin-Conjugating Enzyme E2 T/UBE2T (Ube2t Products)
- Synonyms
- PIG50 Protein, UBE2T Protein, 2700084L22Rik Protein, C80607 Protein, RGD1310816 Protein, hspc150 Protein, wu:fj86h11 Protein, zgc:153687 Protein, ubiquitin conjugating enzyme E2 T Protein, ubiquitin-conjugating enzyme E2T Protein, ubiquitin conjugating enzyme E2T S homeolog Protein, ubiquitin-conjugating enzyme E2T (putative) Protein, UBE2T Protein, Ube2t Protein, ube2t.S Protein, ube2t Protein
- Background
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Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 T, Cell Proliferation-Inducing Gene 50 Protein, Ubiquitin Carrier Protein T, Ubiquitin-Protein Ligase T, UBE2T - Molecular Weight
- 24.7 kDa
- UniProt
- Q9NPD8
- Pathways
- Chromatin Binding
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