VNN1 Protein (AA 22-490) (His tag)

Catalog No. ABIN1097775

Product Details

Target: VNN1 (Vanin 1 (VNN1))

Protein Type: Recombinant

Protein Characteristics: AA 22-490

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This VNN1 protein is labelled with His tag.

Purpose: Recombinant Human Vascular Non-Inflammatory Molecule 1/Vanin-1/VNN1 (C-6His)

Sequence: QDTFTAAVYE HAAILPNATL TPVSREEALA LMNRNLDILE GAITSAADQG AHIIVTPEDA IYGWNFNRDS LYPYLEDIPN PEVNWIPCNN RNRFGQTPVQ ERLSCLAKNN SIYVVANIGD KKPCDTSDPQ CPPDGRYQYN TDVVFDSQGK LVARYHKQNL FMGENQFNVP KEPEIVTFNT TFGSFGIFTC FDILFHDPAV TLVKDFHVDT IVFPTAWMNV LPHLSAVEFH SAWAMGMRVN FLASNIHYPS KKMTGSGIYA PNSSRAFHYD MKTEEGKLLL SQLDSHPSHS AVVNWTSYAS SIEALSSGNK EFKGTVFFDE FTFVKLTGVA GNYTVCQKDL CCHLSYKMSE NIPNEVYALG AFDGLHTVEG RYYLQICTLL KCKTTNLNTC GDSAETASTR FEMFSLSGTF GTQYVFPEVL LSENQLAPGE FQVSTDGRLF SLKPTSGPVL TVTLFGRLYE KDWASNASSV DHHHHHH

Characteristics: Recombinant Human Vascular Non-Inflammatory Molecule 1/Vanin-1 produced by transfected human cells is a secreted protein with sequence (Gln22-Ser490) of Human VNN1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: VNN1 (Vanin 1 (VNN1))

Alternative Name: vanin-1 (VNN1 Products)

Synonyms: HDLCQ8 Protein, Tiff66 Protein, V-1 Protein, hdlcq8 Protein, tiff66 Protein, vnn1.1 Protein, vanin-1 Protein, vanin 1 Protein, pantetheinase Protein, VNN1 Protein, Vnn1 Protein, vnn1 Protein, LOC100217969 Protein

Sub Type: Fusionprotein

Background: Vanin-1 is a cell membrane protein which contains one CN hydrolase domain and belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 is also a member of the Vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. Vanin-1 is widely expressed with higher expression in spleen, kidney and blood and overexpressed in lesional psoriatic skin. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sub-lethal irradiation.ReferencesC spry, et al. Pantothenamides are potent, on-target inhibitors of Plasmodium falciparum growth when serum pantetheinase is inactivated.PMID: 23405100 [PubMed - in process] PMCID: PMC3566143http://www.ncbi.nlm.nih.gov/pubmed/23405100
Alternative Names: Pantetheinase, Pantetheine Hydrolase, Tiff66, Vascular Non-Inflammatory Molecule 1, Vanin-1, VNN1

Molecular Weight: 53.27 kDa

UniProt: O95497

Pathways: Negative Regulation of intrinsic apoptotic Signaling