ZWINT Protein (AA 1-277) (His tag)

Catalog No. ABIN1097843

Product Details

Target: ZWINT (ZW10 Interactor (ZWINT))

Protein Type: Recombinant

Protein Characteristics: AA 1-277

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ZWINT protein is labelled with His tag.

Purpose: Recombinant Human ZW10 Interactor/ZWINT (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDGVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP

Characteristics: Recombinant Human ZW10 Interactor/ZWINT is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro277) of Human ZWINT fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: ZWINT (ZWINT Products)

Synonyms: ZWINT Protein, HZwint-1 Protein, KNTC2AP Protein, ZWINT1 Protein, 2010007E07Rik Protein, 2600001N01Rik Protein, D10Ertd749e Protein, Zwint-1 Protein, Sip30 Protein, ZW10 interacting kinetochore protein Protein, ZW10 interactor Protein, ZWINT Protein, Zwint Protein

Sub Type: Fusionprotein

Background: ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Alternative Names: ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT

Molecular Weight: 33.4 kDa

UniProt: O95229