VPS25 Protein (His tag)
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- Target See all VPS25 Proteins
- VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This VPS25 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product VPS25 Protein
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Vacuolar Protein-Sorting-Associated Protein 25 (VPS25) (AA 1-200) protein (His tag)
VPS25 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Vacuolar Protein-Sorting-Associated Protein 25 (VPS25) (AA 1-176) protein (GST tag)VPS25 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Vacuolar Protein-Sorting-Associated Protein 25 (VPS25) (AA 1-176) protein (Strep Tag)Crystallography grade VPS25 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Vacuolar Protein-Sorting-Associated Protein 25 (VPS25) protein (Myc-DYKDDDDK Tag)VPS25 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Vacuolar protein-sorting-associated protein 25, DERP9, EAP20, FAP20
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1 M Nacl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
- Alternative Name
- VPS25 (VPS25 Products)
- Synonyms
- VPS25 Protein, CG14750 Protein, Dmel\\CG14750 Protein, ESCRT-I Protein, ESCRT-II Protein, dVps25 Protein, l(2)44Db Protein, l(2)k08904 Protein, vps25 Protein, GB16268 Protein, derp9 Protein, eap20 Protein, fap20 Protein, DDBDRAFT_0189483 Protein, DDBDRAFT_0234026 Protein, DDB_0189483 Protein, DDB_0234026 Protein, 1110020N13Rik Protein, AW107467 Protein, D11Wsu68e Protein, im:6898134 Protein, zgc:162560 Protein, DERP9 Protein, EAP20 Protein, FAP20 Protein, vacuolar protein sorting 25 homolog Protein, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene Protein, vacuolar protein sorting-associated protein 25 Protein, WNK lysine deficient protein kinase 4 Protein, Vacuolar protein sorting 25 Protein, vacuolar protein-sorting-associated protein 25 Protein, vacuolar protein sorting 25 homolog L homeolog Protein, vacuolar protein sorting 25 Protein, vacuolar protein sorting 25 homolog (S. cerevisiae) Protein, E2F/DP family winged-helix DNA-binding domain-containing protein Protein, VPS25 Protein, LOC459431 Protein, CpipJ_CPIJ010292 Protein, WNK4 Protein, Vps25 Protein, LOC412381 Protein, vps25.L Protein, vps25 Protein
- Background
- VPS25, also known as vacuolar protein-sorting-associated protein 25, consists of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may play a role in transcription regulation, possibly via its interaction with ELL. Recombinant human VPS25 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.3 kDa(200aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_115729
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